Smolke:Protocols/DO Media

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Recipe

Amino Acid Stock Concentration
(mg/100mL)
Volume Added
(mL/L)
Final Concentration
(mg/L)
Notes
Adenine sulfate 200 100 20 Stored at RT
Uracil 200 100 20
L-Tryptophan 1000 20 20
L-Histidine hydrochloride 1000 20 20
L-Arginine hydrochloride 1000 20 20
L-Methionine 1000 20 20
L-Tyrosine 200 150 30
L-Leucine 1000 60 60
L-Isoleucine 1000 30 30
L-Lysine hydrochloride 1000 30 30
L-Phenylalanine 1000 50 50 Stored at RT
L-Glutamic acid 1000 100 100 Stored at RT
L-Aspartic acid 1000 100 100 Stored at RT
L-Valine 3000 50 150
L-Threonine 4000 50 200
L-Serine 8000 50 400
Nanopure Water Remainder

Notes

  • Leave out whichever amino acid(s) you're dropping out, and replace the volume with water.
  • Stocks which are stored at RT are in the cabinet with autoclaved media.

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