Smolke:Protocols/DO Media

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Recipe Amino Acid Stock Concentration (g/L) Volume Added (mL/L) Final Media Concentration (mg/L) Notes Adenine sulfate 2 100 20 Uracil 2 100 20 L-Tryptophan 10 20 20 L-Histidine hydrochloride 10 20 20 L-Arginine hydrochloride 10 20 20 L-Methionine 10 20 20 L-Tyrosine 2 150 30 add ~5mL 5N NaOH L-Leucine 10 60 60 L-Isoleucine 10 30 30 L-Lysine hydrochloride 10 30 30 L-Phenylalanine 10 50 50 L-Glutamic acid 10 100 100 Stored at RT L-Aspartic acid 10 100 100 Stored at RT L-Valine 30 50 150 L-Threonine 40 50 200 L-Serine 80 50 400 Nanopure Water Remainder Notes Leave out whichever amino acid(s) you're dropping out, and replace the volume with water. Stocks which are stored at RT are in the cabinet with autoclaved media. Contact Stephanie