Smolke:Protocols/DO Media: Difference between revisions
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==Recipe== | ==Recipe== | ||
{| border="1" class="wikitable" style="text-align:center" | {| border="1" class="wikitable" style="text-align:center" | ||
! Amino Acid !! Stock Concentration<br>( | ! Amino Acid !! Stock Concentration<br>(g/L) !! Volume Added<br>(mL/L) !! Final Media Concentration <br>(mg/L) !! Notes | ||
|- | |- | ||
! Adenine sulfate | ! Adenine sulfate | ||
| | | 2 || 100 || 20 || | ||
|- | |- | ||
! Uracil | ! Uracil | ||
| | | 2 || 100 || 20 || | ||
|- | |- | ||
! L-Tryptophan | ! L-Tryptophan | ||
| | | 10 || 20 || 20 || | ||
|- | |- | ||
! L-Histidine hydrochloride | ! L-Histidine hydrochloride | ||
| | | 10 || 20 || 20 || | ||
|- | |- | ||
! L-Arginine hydrochloride | ! L-Arginine hydrochloride | ||
| | | 10 || 20 || 20 || | ||
|- | |- | ||
! L-Methionine | ! L-Methionine | ||
| | | 10 || 20 || 20 || | ||
|- | |- | ||
! L-Tyrosine | ! L-Tyrosine | ||
| | | 2 || 150 || 30 || add ~5mL 5N NaOH | ||
|- | |- | ||
! L-Leucine | ! L-Leucine | ||
| | | 10 || 60 || 60 || | ||
|- | |- | ||
! L-Isoleucine | ! L-Isoleucine | ||
| | | 10 || 30 || 30 || | ||
|- | |- | ||
! L-Lysine hydrochloride | ! L-Lysine hydrochloride | ||
| | | 10 || 30 || 30 || | ||
|- | |- | ||
! L-Phenylalanine | ! L-Phenylalanine | ||
| | | 10 || 50 || 50 || | ||
|- | |- | ||
! L-Glutamic acid | ! L-Glutamic acid | ||
| | | 10 || 100 || 100 || Stored at RT | ||
|- | |- | ||
! L-Aspartic acid | ! L-Aspartic acid | ||
| | | 10 || 100 || 100 || Stored at RT | ||
|- | |- | ||
! L-Valine | ! L-Valine | ||
| | | 30 || 50 || 150 || | ||
|- | |- | ||
! L-Threonine | ! L-Threonine | ||
| | | 40 || 50 || 200 || | ||
|- | |- | ||
! L-Serine | ! L-Serine | ||
| | | 80 || 50 || 400 || | ||
|- | |- | ||
! Nanopure Water | ! Nanopure Water | ||
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==Contact== | ==Contact== | ||
[[User: | [[User:Stephanie Galanie|Stephanie]] |
Latest revision as of 14:27, 8 August 2014
Recipe
Notes
Contact |