Smolke:Protocols/DO Media

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Recipe

Amino Acid Stock Concentration
(g/L)
Volume Added
(mL/L)
Final Concentration
(mg/L)
Notes
Adenine sulfate 2 100 20
Uracil 2 100 20
L-Tryptophan 10 20 20
L-Histidine hydrochloride 10 20 20
L-Arginine hydrochloride 10 20 20
L-Methionine 10 20 20
L-Tyrosine 2 150 30 add ~5mL 5N NaOH
L-Leucine 10 60 60
L-Isoleucine 10 30 30
L-Lysine hydrochloride 10 30 30
L-Phenylalanine 10 50 50
L-Glutamic acid 10 100 100 Stored at RT
L-Aspartic acid 10 100 100 Stored at RT
L-Valine 30 50 150
L-Threonine 40 50 200
L-Serine 80 50 400
Nanopure Water Remainder

Notes

  • Leave out whichever amino acid(s) you're dropping out, and replace the volume with water.
  • Stocks which are stored at RT are in the cabinet with autoclaved media.

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