Smolke:Protocols/DO Media

From OpenWetWare

< Smolke:Protocols(Difference between revisions)
Jump to: navigation, search
(Contact)
Current revision (16:27, 8 August 2014) (view source)
(Recipe)
 
Line 3: Line 3:
==Recipe==
==Recipe==
{| border="1" class="wikitable" style="text-align:center"
{| border="1" class="wikitable" style="text-align:center"
-
! Amino Acid !! Stock Concentration<br>(g/L) !! Volume Added<br>(mL/L) !! Final Concentration<br>(mg/L) !! Notes
+
! Amino Acid !! Stock Concentration<br>(g/L) !! Volume Added<br>(mL/L) !! Final Media Concentration <br>(mg/L) !! Notes
|-
|-
! Adenine sulfate  
! Adenine sulfate  

Current revision

Home        Contact        Internal        Protocols        Lab Members        Publications        Research       


Recipe

Amino Acid Stock Concentration
(g/L)
Volume Added
(mL/L)
Final Media Concentration
(mg/L)
Notes
Adenine sulfate 2 100 20
Uracil 2 100 20
L-Tryptophan 10 20 20
L-Histidine hydrochloride 10 20 20
L-Arginine hydrochloride 10 20 20
L-Methionine 10 20 20
L-Tyrosine 2 150 30 add ~5mL 5N NaOH
L-Leucine 10 60 60
L-Isoleucine 10 30 30
L-Lysine hydrochloride 10 30 30
L-Phenylalanine 10 50 50
L-Glutamic acid 10 100 100 Stored at RT
L-Aspartic acid 10 100 100 Stored at RT
L-Valine 30 50 150
L-Threonine 40 50 200
L-Serine 80 50 400
Nanopure Water Remainder

Notes

  • Leave out whichever amino acid(s) you're dropping out, and replace the volume with water.
  • Stocks which are stored at RT are in the cabinet with autoclaved media.

Contact

Stephanie

Personal tools