Smolke:Lab Jobs: Difference between revisions
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'''Andy''' | '''Andy''' | ||
:plasmid prep buffers (P1, P2, N3, PE, EB, stocks), | :plasmid prep buffers (P1, P2, N3, PE, EB, stocks - confirm with testing) | ||
:'' | :'''' | ||
'''Ben''' | |||
:-T plates (II), -U plates (II) | |||
:''4 refrigerator/cold room organization'' | |||
'''Christina''' | '''Christina''' | ||
:''general lab ordering'' | :''general lab ordering'' | ||
'''Julie''' | '''Julie''' | ||
Line 23: | Line 19: | ||
'''Isis''' | '''Isis''' | ||
:LB+amp plates ( | :LB+amp plates (III) | ||
:'' | :''journal club, secondary lab safety officer'' | ||
'''Jay''' | '''Jay''' | ||
:10x sugar stocks (dextrose), 60% glycerol | :10x sugar stocks (20% dextrose), 60% glycerol | ||
:'' | :'''' | ||
'''Joy''' | |||
:LB+amp plates (II) | |||
:''computer administrator and databases (back up)'' | |||
'''Kate''' | '''Kate''' | ||
Line 35: | Line 35: | ||
'''Kathy''' | '''Kathy''' | ||
: | :competent cells (II), general cleaning of high speed centrifuge | ||
:''Quanta, MACSQuant'' | :''Quanta, MACSQuant'' | ||
'''Maureen''' | '''Maureen''' | ||
:sodium acetate, TAE stocks, 10x drop out stocks for plates (II), -UT plates (II) | :sodium acetate, TAE stocks, 10x drop out stocks for plates (II), -UT plates (II) | ||
:'' | :''radioactivity safety officer, Biacore'' | ||
'''Melina''' | '''Melina''' | ||
: | :Gibson master mix, antibiotics | ||
:'' | :''lab wiki, TC Room (II)'' | ||
'''Mike S''' | |||
:-T plates (I), YPD plates, YPD [[http://openwetware.org/wiki/Smolke:Protocols/Yeast_Media_Recipes]] | |||
:''LC-IT'' | |||
'''Michael R''' | |||
:LB+amp plates (I) | |||
:''cryogenic storage LN2 refill (I)'' | |||
''' | '''Peter''' | ||
: | :LB, pH meters, aliquot dNTPs, water baths (shared with Endy lab) | ||
:'' | :''TC Room; cryogenic storage LN2 refill (II)'' | ||
'''Remus''' | '''Remus''' | ||
:sterile water, EDTA stocks, TC room water bath | :sterile water, EDTA stocks, TC room water bath | ||
:''general enzyme ordering | :''general enzyme ordering'' | ||
''' | '''Shireen''' | ||
: | :competent cells (I) | ||
:'' | :''freezer (-80/-20)'' | ||
'''Stephanie''' | '''Stephanie''' | ||
:amino acid stocks, 10x drop out stocks for plates (I) | :amino acid stocks, 10x drop out stocks for plates (I) | ||
:''LC | :''LC-QQQ, inventory chemical waste bottles/containers (fume hood)'' | ||
'''Yanran''' | |||
:YNB/NINR [[http://openwetware.org/wiki/Smolke:Protocols/Yeast_Media_Recipes]], SOC | |||
:''semiprep-LC'' | |||
'''Yen-Hsiang''' | '''Yen-Hsiang''' | ||
: | :TBD | ||
:''computer administrator and databases'' | :''computer administrator and databases'' | ||
Revision as of 13:55, 10 June 2014
Laboratory Jobs and Administrative AssignmentsAndy
Ben
Christina
Julie
Isis
Jay
Joy
Kate
Kathy
Maureen
Melina
Mike S
Michael R
Peter
Remus
Shireen
Stephanie
Yanran
Yen-Hsiang
Notes
How It WorksEach person is assigned a laboratory job (or set of jobs based on intensity of the assigned job) and an administrative role (indicated in italics). Please keep up with your assigned jobs. Note, that it is everyone's responsibility to load tips, load the dishwasher, and rinse out your glassware. Also, while general DO plates are made (-U, -T, and -UT) as common stocks, all other DO plates are made by the individual using them. |