Smolke:Lab Jobs

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(Laboratory Jobs and Administrative Assignments)
(Laboratory Jobs and Administrative Assignments)
(4 intermediate revisions not shown.)
Line 4: Line 4:
'''Andy'''           
'''Andy'''           
-
:plasmid prep buffers (P1, P2, N3, PE, EB, stocks), aliquot dNTPs (II)
+
:plasmid prep buffers (P1, P2, N3, PE, EB, stocks - confirm with testing)
:''Biacore''
:''Biacore''
Line 11: Line 11:
'''Drew'''
'''Drew'''
-
: electrocompetent cells (I), water baths
+
: electrocompetent cells (I), water baths (shared with Endy lab)
:''4 refrigerator/cold room organization; freezer (-80/-20)''
:''4 refrigerator/cold room organization; freezer (-80/-20)''
-
 
-
'''Eric'''
 
-
:LB (I), pH meters, aliquot dNTPs (I)
 
-
:''journal club''
 
'''Julie'''
'''Julie'''
Line 24: Line 20:
'''Isis'''
'''Isis'''
:LB+amp plates (II)
:LB+amp plates (II)
-
:''databases and software, secondary lab safety officer''
+
:''software, secondary lab safety officer''
'''Jay'''
'''Jay'''
-
:10x sugar stocks (dextrose), TAE stocks, 60% glycerol, sodium acetate
+
:10x sugar stocks (20% dextrose), 60% glycerol
:''CE, cryogenic storage LN2 refill (I)''
:''CE, cryogenic storage LN2 refill (I)''
-
'''Joe'''
+
'''Joy'''
-
:amino acid stocks, 10x drop out stocks for plates (I)
+
:LB+amp plates (III)
-
:''general enzyme ordering and information''
+
:''computer administrator and databases (back up)''
'''Kate'''
'''Kate'''
Line 42: Line 38:
:''Quanta, MACSQuant''
:''Quanta, MACSQuant''
-
'''Leo'''
+
'''Maureen'''
-
:LB+amp plates (I)
+
:sodium acetate, TAE stocks, 10x drop out stocks for plates (II), -UT plates (II)
:''radioactivity safety officer''
:''radioactivity safety officer''
'''Melina'''
'''Melina'''
:LB (II), antibiotics
:LB (II), antibiotics
-
:''lab wiki''
+
:''lab wiki, TC Room (II)''
'''Michael'''
'''Michael'''
Line 56: Line 52:
'''Remus'''
'''Remus'''
:sterile water, EDTA stocks, TC room water bath
:sterile water, EDTA stocks, TC room water bath
-
:''general enzyme ordering, TC Room (II)''
+
:''general enzyme ordering''
'''Ryan'''
'''Ryan'''
-
:LB+amp plates (III)
+
:LB+amp plates (II)
:''TC Room; cryogenic storage LN2 refill (II)''
:''TC Room; cryogenic storage LN2 refill (II)''
Line 65: Line 61:
:amino acid stocks, 10x drop out stocks for plates (I)
:amino acid stocks, 10x drop out stocks for plates (I)
:''LC and fraction collector, inventory chemical waste bottles/containers (fume hood)''
:''LC and fraction collector, inventory chemical waste bottles/containers (fume hood)''
 +
 +
'''Yanran'''
 +
:LB (I), pH meters, aliquot dNTPs
 +
:''journal club''
'''Yen-Hsiang'''           
'''Yen-Hsiang'''           
-
:YNB [[http://openwetware.org/wiki/Smolke:Protocols/Yeast_Media_Recipes]], NINR, -UT plates (II), SOC
+
:YNB [[http://openwetware.org/wiki/Smolke:Protocols/Yeast_Media_Recipes]], NINR, SOC
:''computer administrator and databases''
:''computer administrator and databases''

Revision as of 00:57, 23 July 2013

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Laboratory Jobs and Administrative Assignments

Andy

plasmid prep buffers (P1, P2, N3, PE, EB, stocks - confirm with testing)
Biacore

Christina

general lab ordering

Drew

electrocompetent cells (I), water baths (shared with Endy lab)
4 refrigerator/cold room organization; freezer (-80/-20)

Julie

glass beads, toothpicks, used plate sterilization, glass/plastic washing sterilization, disposable sterilization
safety officer, general lab organization/maintenance, sharps, cleaning general areas, order inventory, inventory MSDS sheets, organize library

Isis

LB+amp plates (II)
software, secondary lab safety officer

Jay

10x sugar stocks (20% dextrose), 60% glycerol
CE, cryogenic storage LN2 refill (I)

Joy

LB+amp plates (III)
computer administrator and databases (back up)

Kate

-U plates, -UT plates (I)
schedule II officer, general enzyme ordering and information (back up)

Kathy

electrocompetent cells (II), general cleaning of high speed centrifuge
Quanta, MACSQuant

Maureen

sodium acetate, TAE stocks, 10x drop out stocks for plates (II), -UT plates (II)
radioactivity safety officer

Melina

LB (II), antibiotics
lab wiki, TC Room (II)

Michael

-T plates, YPD plates, YPD [[1]]
LC-MS

Remus

sterile water, EDTA stocks, TC room water bath
general enzyme ordering

Ryan

LB+amp plates (II)
TC Room; cryogenic storage LN2 refill (II)

Stephanie

amino acid stocks, 10x drop out stocks for plates (I)
LC and fraction collector, inventory chemical waste bottles/containers (fume hood)

Yanran

LB (I), pH meters, aliquot dNTPs
journal club

Yen-Hsiang

YNB [[2]], NINR, SOC
computer administrator and databases


Notes

  • Please make sure BEFORE you leave for a vacation that you check on stocks (or whatever is appropriate) for your lab job. Make sure you anticipate usage over the time you will be out of the lab and that there will be enough stocks, etc to last the lab before you return. If you are concerned about lab usage while you are gone, it is your responsibility to ask someone in the lab to monitor it for you and cover while you are away.
  • General lab courtesy - if you use a whole sleeve of plates, make another. If you use a lot of a stock or finish it, make some more.
  • Everyone should help aliquot electrocomp cells.
  • Solution I and Solution II are now the responsibility of those who use them regularly. TBE is also the responsibility of those who are using it regularly.

How It Works

Each person is assigned a laboratory job (or set of jobs based on intensity of the assigned job) and an administrative role (indicated in italics). Please keep up with your assigned jobs. Note, that it is everyone's responsibility to load tips, load the dishwasher, and rinse out your glassware. Also, while general DO plates are made (-U, -T, and -UT) as common stocks, all other DO plates are made by the individual using them.

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