Smolke:Enzyme inventory: Difference between revisions

Revision as of 21:31, 2 September 2010

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Enzyme	Regular	HF	Regular Backup	HF Backup
AgeI		Y		Y
AleI		Y		Y
ApaI		Y		Y
BamHI		Y		Y
DpnI	Y		Y
EcoRI	Y	Y		Y
EcoRV		Y		Y
HindIII	Y		Y
MfeI		Y		Y
NotI	Y	Y		Y
SacI		Y		Y
SacII	Y		Y
SaII	Y		Y
XbaI	Y		Y
XhoI	Y		Y

When the working stock has been (or almost) used up, please replace (or combine) with a new tube instead of leaving the empty (or almost empty) tube in the cooler.
What is high fidelity (HF) restriction enzyme?
Double digest finder

@@ Line 5: / Line 5: @@
 ! Enzyme !! Regular !! HF !! Regular Backup !! HF Backup
 |-
-! AgeI || Yes || Yes
+! AgeI || ||Y|| ||Y
 |-
-! AleI || Yes ||
+! AleI || ||Y|| ||Y
 |-
-! ApaI || Yes ||
+! ApaI || ||Y|| ||Y
 |-
-! BamHI || Yes || Yes
+! BamHI || ||Y|| ||Y
 |-
-! DpnI || Yes ||
+! DpnI ||Y|| ||Y||
 |-
-! EcoRI || Yes || Yes
+! EcoRI ||Y||Y|| ||Y
 |-
-! EcoRV || Yes || Yes
+! EcoRV || ||Y|| ||Y
 |-
-! HindIII || Yes ||
+! HindIII ||Y|| ||Y||
 |-
-! MfeI || Yes || Yes
+! MfeI || ||Y|| ||Y
 |-
-! NotI || Yes || Yes
+! NotI ||Y||Y|| ||Y
 |-
-! SacI || Yes || Yes
+! SacI || ||Y|| ||Y
 |-
-! SacII || Yes ||
+! SacII || Y || ||Y||
 |-
-! SaII || Yes || Yes
+! SaII ||Y|| ||Y||
 |-
-! XbaI || Yes ||
+! XbaI ||Y|| ||Y||
 |-
-! XhoI || Yes ||
+! XhoI ||Y|| ||Y||
 |-
 |}