Silver: Protocols

See also the OpenWetWare Protocols Collection.

Silver Lab Members: Link to your protocols from both this page and the OpenWetWare Protocols page.

Back to Silver Lab

Genome Organization

• S. cerevisiae Protocols
  • ChIP-chip
  • FISH/IF
  • Immunofluorescence
  • LiOAc Transformation
  • Propidium Iodide FACS
• Mammalian Protocols

RNA

• Poly A RNA in situ protocol
• RNA Export Assay

Synthetic Biology

• Strategy for Making Parts
• Commonly Used Plasmids
• Commonly Used Primers
• PCR
• Oligonucleotide Inserts
• Overlapping Oligonucleotide Inserts
• Site-Directed Mutagenesis
• Restriction Digest
• Ligation
• Quick & Dirty Digest & Ligation
• Bacterial Transformation
• Plasmid Verification
• Integration into Yeast
• Verifying Genomic Integration

Cell-Based Screens

• Chemical compound screens
• miRNA screens
• High-throughput immunostaining
• FKHR Translocation Assay

Methylation

• Methylation Assay
• In Vivo Protein Methylation Assay (S. cerevisiae)

Other / More General

S. cerevisiae Protocols

• Lysate for Western
• Colony PCR
• Time-Lapse Microscopy

Mammamlian Tissue Culture Protocols

• Colony picking using cloning rings

Other Protocols

• Bioptechs live-cell micro-observation perfusion chambers
• Cleaning Glass Beads

Common Reagents

• Ampicillin
• B Plates
• Coomassie Stain
• Destain
• Dropout Media
• Dropout Plates
• 0.5 M EDTA pH 8.0
• 20% Glucose
• 50% Glycerol
• LB Liquid
• LB-Amp Plates
• 10x PBS
• Protease Inhibitors
• ssDNA
• SOC
• 50x TAE
• 1M Tris Base
• TE
• YEPD Liquid
• YEPD Plates

Hardware_Maintenance

• Microscope

Software

[[Media: |TALE Software]]