Silver: Protocols: Difference between revisions
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==Synthetic Biology== | ==Synthetic Biology== | ||
*[[Silver: BB Strategy | Strategy for Making Parts]] | *[[Silver: BB Strategy | Strategy for Making Parts]] | ||
*[[Silver: Commonly Used Plasmids | Commonly Used Plasmids]] | |||
*[[Silver: Commonly Used Primers | Commonly Used Primers]] | |||
*[[Silver: PCR | PCR]] | *[[Silver: PCR | PCR]] | ||
*[[Silver: Oligonucleotide Inserts | Oligonucleotide Inserts]] | *[[Silver: Oligonucleotide Inserts | Oligonucleotide Inserts]] | ||
*[[Silver: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]] | *[[Silver: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]] | ||
*[[Silver: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | |||
*[[Silver: Restriction Digest | Restriction Digest]] | *[[Silver: Restriction Digest | Restriction Digest]] | ||
*[[Silver: Ligation | Ligation]] | *[[Silver: Ligation | Ligation]] | ||
*[[Silver: Quick & Dirty Digest & Ligation | Quick & Dirty Digest & Ligation]] | |||
*[[Silver: Bacterial Transformation | Bacterial Transformation]] | *[[Silver: Bacterial Transformation | Bacterial Transformation]] | ||
*[[Silver: Plasmid Verification | Plasmid Verification]] | *[[Silver: Plasmid Verification | Plasmid Verification]] | ||
| Line 46: | Line 49: | ||
*[[Silver: Colony PCR|Colony PCR]] | *[[Silver: Colony PCR|Colony PCR]] | ||
*[[Silver: Time-Lapse Microscopy|Time-Lapse Microscopy]] | *[[Silver: Time-Lapse Microscopy|Time-Lapse Microscopy]] | ||
=== Mammamlian Tissue Culture Protocols=== | |||
*[[Silver: Colony picking using cloning rings|Colony picking using cloning rings]] | |||
===Other Protocols=== | ===Other Protocols=== | ||
*[[LAB Media]] - Lithium Acetate Borate electrophoresis media | |||
*[http://www.bioptechs.com/ Bioptechs live-cell micro-observation perfusion chambers] | *[http://www.bioptechs.com/ Bioptechs live-cell micro-observation perfusion chambers] | ||
*[[Silver: Cleaning Glass Beads|Cleaning Glass Beads]] | *[[Silver: Cleaning Glass Beads|Cleaning Glass Beads]] | ||
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*[[Silver: Dropout Media|Dropout Media]] | *[[Silver: Dropout Media|Dropout Media]] | ||
*[[Silver: Dropout Plates|Dropout Plates]] | *[[Silver: Dropout Plates|Dropout Plates]] | ||
*[[Silver: EDTA|0.5 M EDTA pH 8.0]] | *[[Silver: EDTA|0.5 M EDTA pH 8.0]] | ||
*[[Silver: 20% Glucose|20% Glucose]] | *[[Silver: 20% Glucose|20% Glucose]] | ||
*[[Silver: 50% Glycerol|50% Glycerol]] | *[[Silver: 50% Glycerol|50% Glycerol]] | ||
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*[[Silver: LB-Amp Plates|LB-Amp Plates]] | *[[Silver: LB-Amp Plates|LB-Amp Plates]] | ||
*[[Silver: 10x PBS|10x PBS]] | *[[Silver: 10x PBS|10x PBS]] | ||
*[[Silver: Protease Inhibitors|Protease Inhibitors]] | |||
*[[Silver: ssDNA|ssDNA]] | |||
*[[Silver: SOC|SOC]] | *[[Silver: SOC|SOC]] | ||
*[[Silver: 50x TAE|50x TAE]] | *[[Silver: 50x TAE|50x TAE]] | ||
*[[Silver: 1M Tris Base|1M Tris Base]] | *[[Silver: 1M Tris Base|1M Tris Base]] | ||
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*[[Silver: YEPD Liquid|YEPD Liquid]] | *[[Silver: YEPD Liquid|YEPD Liquid]] | ||
*[[Silver: YEPD Plates|YEPD Plates]] | *[[Silver: YEPD Plates|YEPD Plates]] | ||
==Hardware_Maintenance== | |||
*[[Silver: Microscope|Microscope]] | |||
===Software=== | |||
[[Media: |TALE Software]] | |||
Latest revision as of 10:25, 6 June 2013
See also the OpenWetWare Protocols Collection.
Silver Lab Members: Link to your protocols from both this page and the OpenWetWare Protocols page.
Back to Silver Lab
Genome Organization
- S. cerevisiae Protocols
- Mammalian Protocols
RNA
Synthetic Biology
- Strategy for Making Parts
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
- Integration into Yeast
- Verifying Genomic Integration
Cell-Based Screens
Methylation
Other / More General
S. cerevisiae Protocols
Mammamlian Tissue Culture Protocols
Other Protocols
- LAB Media - Lithium Acetate Borate electrophoresis media
- Bioptechs live-cell micro-observation perfusion chambers
- Cleaning Glass Beads
Common Reagents
- Ampicillin
- B Plates
- Coomassie Stain
- Destain
- Dropout Media
- Dropout Plates
- 0.5 M EDTA pH 8.0
- 20% Glucose
- 50% Glycerol
- LB Liquid
- LB-Amp Plates
- 10x PBS
- Protease Inhibitors
- ssDNA
- SOC
- 50x TAE
- 1M Tris Base
- TE
- YEPD Liquid
- YEPD Plates
Hardware_Maintenance
Software
[[Media: |TALE Software]]
