Silver: B plates: Difference between revisions

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Latest revision as of 08:37, 5 July 2006

B Plates (per L)

  • In 2L flask:
    • 6.7 g bacto yeast extract (w/o amino acids)
    • 20g agar
    • 900 mL H2O
  • Autoclave, and also autoclave a stir bar in a separate beaker
  • Add stir bar and 100 mL of 20% glucose.
  • Stir until warm to the touch, and pour plates.