Silver: 50x TAE

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
(categories)
(2 intermediate revisions not shown.)
Line 1: Line 1:
 +
The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA.
 +
Per 1L:
Per 1L:
*242g Tris base
*242g Tris base
-
*57.1 ml acetic acid
+
*57.1 ml acetic acid (glacial)
*100ml 0.5 M EDTA
*100ml 0.5 M EDTA
'''DO NOT AUTOCLAVE'''
'''DO NOT AUTOCLAVE'''
 +
 +
[[Category:Material]]
 +
[[Category:Buffer]]
 +
[[Category:Agarose gel electrophoresis]]

Revision as of 08:02, 5 May 2009

The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA.

Per 1L:

  • 242g Tris base
  • 57.1 ml acetic acid (glacial)
  • 100ml 0.5 M EDTA

DO NOT AUTOCLAVE

Personal tools