Shreffler:Standard Buffers Solutions Media: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 10: Line 10:
== Media ==
== Media ==


=== [[LB]]: ===
=== [[LB]] ===
 
'''Luria Bertani Media'''


Modified to make 0.5 L
Modified to make 0.5 L

Revision as of 18:39, 27 May 2009

Many of these are available at Materials and to reduce redundancy those are referred to and this page is not included in the Protocols or Materials categories.

This page contains lab-specific details -- please edit.


Buffers

Phosphate Buffered Saline (PBS)

Media

LB

Luria Bertani Media

Modified to make 0.5 L

  • Dissolve:
    • 5g of tryptone,
    • 2.5g of yeast extract, and
    • 5g of NaCl in 450 mL of dH2O
  • Place on stirring plate with stir bar and allow to mix, until everything dissolves
  • After completely dissolved, bring the volume up to 500 mL
  • Divide into two 150 ml flasks and store remaining in a sterile bottle
  • Autoclave the two flasks and sterile bottle (30 min)

Other

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Shreffler:Standard_Buffers_Solutions_Media&oldid=310577"