Shreffler:Notebook/Alex Daily/2012/04/04: Difference between revisions
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==Sarita Buffy== | ==Sarita Buffy== | ||
Sarita received buffy coat from MGH (from Tues 4/3/12). I ficolled/counted (550 million PBMCs) and stripped ~400 million cells with lactic acid following Sarita's lactic acid stripping protocol. These were washed twice and resuspended in 800 µl RPMI. 400 µl were passively sensitized with serum drawn from PNOIT #965-03 (high IgE levels for Ara h 2 according to phadia results); the other 400 µl were sensitized with Sarita's own serum as a negative control for peanut-specific response. | *Sarita received buffy coat from MGH (from Tues 4/3/12). I ficolled/counted (550 million PBMCs) and stripped ~400 million cells with lactic acid following Sarita's lactic acid stripping protocol. These were washed twice and resuspended in 800 µl RPMI. 400 µl were passively sensitized with serum drawn from PNOIT #965-03 (high IgE levels for Ara h 2 according to phadia results); the other 400 µl were sensitized with Sarita's own serum as a negative control for peanut-specific response. | ||
Other 150 million cells used in Sarita's plasmablast staining. | *Other 150 million cells used in Sarita's plasmablast staining. | ||
==TCR Sequencing== | |||
The transfected cells that we plated yesterday | |||
Revision as of 11:53, 4 April 2012
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Sarita Buffy
TCR SequencingThe transfected cells that we plated yesterday | |
