Shreffler:Notebook/Alex Daily/2012/03/21: Difference between revisions
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Ran SDS-PAGE with Bert to gauge purity/protein concentration of Ara h 2, Ara h 3, and Ara h 6 stock from Mt Sinai, each with a stated concentration of 3 mg/ml. Ran nanodrop to determine true (approximate) protein concentrations: | |||
Ara h 2: ~1 mg/ml | |||
Ara h 3: ~2 mg/ml | |||
Ara h 6: ~0.5 mg/ml | |||
For SDS-PAGE, we want to create a loading solution for each protein at a concentration of 0.5 µg/µl. | |||
Loading solution composition: | |||
Loaded the gel w/ 1 µg and 5 µg for each protein. | |||
Gel | |||
According to Bert, band sizes, when matched to bands of known protein concentration (from his past gels), reflected nanodrop concentrations fairly well. | |||
Revision as of 08:55, 22 March 2012
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Entry title
Ran SDS-PAGE with Bert to gauge purity/protein concentration of Ara h 2, Ara h 3, and Ara h 6 stock from Mt Sinai, each with a stated concentration of 3 mg/ml. Ran nanodrop to determine true (approximate) protein concentrations: Ara h 2: ~1 mg/ml Ara h 3: ~2 mg/ml Ara h 6: ~0.5 mg/ml For SDS-PAGE, we want to create a loading solution for each protein at a concentration of 0.5 µg/µl. Loading solution composition: Loaded the gel w/ 1 µg and 5 µg for each protein. Gel According to Bert, band sizes, when matched to bands of known protein concentration (from his past gels), reflected nanodrop concentrations fairly well.
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