Shreffler:Eosinophil Isolation: Difference between revisions
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(New page: '''Granulocyte Isolation''': # Under hood, spray down the buffy bag and scissors with 70% EtOH and wait until dry. Then cut oopen the buffy coat bag and pour blood into a 50 mL Falcon tube...) |
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==Overview== | |||
# Under hood, spray down the buffy | |||
Isolation of eosinophils for migration studies in collaboration with Bryan Hurley | |||
==Materials== | |||
* | |||
==Procedure== | |||
# Under hood, spray down the buffy bags and scissors with 70% EtOH and wait until dry. | |||
# Cut open the buffy coat bag and pur blood into a 50 mL Falcon tube. | |||
==Discussion== | |||
[[Talk:{{PAGENAME}}|discuss this protocol]] | |||
==References== | |||
==Contact== | |||
*Who has experience with this protocol? | |||
*[[Special:Emailuser/Wayne G. Shreffler|Email Wayne Shreffler through OpenWetWare]] | |||
[[Category:ShreffLab]] | |||
[[Category:Protocol]] | |||
[[Category:Flow cytometry]] | |||
[[Category:Immunology]] | |||
Latest revision as of 10:29, 4 May 2011
Overview
Isolation of eosinophils for migration studies in collaboration with Bryan Hurley
Materials
Procedure
- Under hood, spray down the buffy bags and scissors with 70% EtOH and wait until dry.
- Cut open the buffy coat bag and pur blood into a 50 mL Falcon tube.
Discussion
References
Contact
- Who has experience with this protocol?
- Email Wayne Shreffler through OpenWetWare
