Shreffler:Eosinophil Isolation

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Current revision (12:29, 4 May 2011) (view source)
 
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'''Granulocyte Isolation''':
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==Overview==
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# Under hood, spray down the buffy bag and scissors with 70% EtOH and wait until dry. Then cut open the buffy coat bag and pour blood into a 50 mL Falcon tube.
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Isolation of eosinophils for migration studies in collaboration with Bryan Hurley
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==Materials==
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*
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==Procedure==
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# Under hood, spray down the buffy bags and scissors with 70% EtOH and wait until dry.
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# Cut open the buffy coat bag and pur blood into a 50 mL Falcon tube.
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==Discussion==
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[[Talk:{{PAGENAME}}|discuss this protocol]]
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==References==
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==Contact==
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*Who has experience with this protocol?
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*[[Special:Emailuser/Wayne G. Shreffler|Email Wayne Shreffler through OpenWetWare]]
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[[Category:ShreffLab]]
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[[Category:Protocol]]
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[[Category:Flow cytometry]]
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[[Category:Immunology]]

Current revision

Contents

Overview

Isolation of eosinophils for migration studies in collaboration with Bryan Hurley

Materials

Procedure

  1. Under hood, spray down the buffy bags and scissors with 70% EtOH and wait until dry.
  2. Cut open the buffy coat bag and pur blood into a 50 mL Falcon tube.

Discussion

discuss this protocol

References

Contact

Personal tools