Sequencing DNA: Difference between revisions
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==Specific Protocols== | ==Specific Protocols== | ||
[[ | [[Sequencing BioBrick DNA at MIT]] -- protocol for DNA sequencing of BioBrick parts | ||
==Notes== | ==Notes== | ||
At least at the biopolymers facility and probably true for most sequencing results, when doing a run-off sequencing reaction, an extra (sometimes pretty high amplitude) 'A' peak is seen at the end before the template ends. The workers at the biopolymers facility seemed surprised when they were told about this, but they were able to find out that the sequenase enzyme used in the sequencing reaction is a genetically modified form of Taq. Therefore, it is most probable that the extra A is the template-independent A that Taq tends to add to the 3'-end of DNA. | At least at the biopolymers facility and probably true for most sequencing results, when doing a run-off sequencing reaction, an extra (sometimes pretty high amplitude) 'A' peak is seen at the end before the template ends. The workers at the biopolymers facility seemed surprised when they were told about this, but they were able to find out that the sequenase enzyme used in the sequencing reaction is a genetically modified form of Taq. Therefore, it is most probable that the extra A is the template-independent A that Taq tends to add to the 3'-end of DNA. | ||
Revision as of 15:22, 3 December 2005
Specific Protocols
Sequencing BioBrick DNA at MIT -- protocol for DNA sequencing of BioBrick parts
Notes
At least at the biopolymers facility and probably true for most sequencing results, when doing a run-off sequencing reaction, an extra (sometimes pretty high amplitude) 'A' peak is seen at the end before the template ends. The workers at the biopolymers facility seemed surprised when they were told about this, but they were able to find out that the sequenase enzyme used in the sequencing reaction is a genetically modified form of Taq. Therefore, it is most probable that the extra A is the template-independent A that Taq tends to add to the 3'-end of DNA.
