Sean Lauber:Using the Kras G12D Mouse Model for Lung Cancer: Difference between revisions
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(New page: This procedure involves first precipitating adenovirus before it is administered by intubation. For 1000 ul: CaCl2 2 ul 10X EMEM 100 ul 1. Calculate the appr...) |
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| Line 2: | Line 2: | ||
For 1000 ul: | For 1000 ul: | ||
CaCl2 2 ul | 2 M CaCl2 2 ul | ||
10X EMEM | 10X EMEM 100 ul | ||
0.2 M L-glut 10 ul | |||
Virus x ul | |||
Sterile water 888-x ul | |||
1. Calculate the appropriate amount of virus to | 1. Calculate the appropriate amount of virus and add it (without CaCl2) along with the other reagents to an ependorff | ||
2. | 2. 20 minutes before you plan on intubating, add the CaCl2 and vortex | ||
3. Use within 1 hour (after 20 minutes of waiting) | |||
Latest revision as of 12:49, 18 July 2013
This procedure involves first precipitating adenovirus before it is administered by intubation.
For 1000 ul:
2 M CaCl2 2 ul
10X EMEM 100 ul
0.2 M L-glut 10 ul
Virus x ul
Sterile water 888-x ul
1. Calculate the appropriate amount of virus and add it (without CaCl2) along with the other reagents to an ependorff
2. 20 minutes before you plan on intubating, add the CaCl2 and vortex
3. Use within 1 hour (after 20 minutes of waiting)
