Sean Lauber:Isotype ELISA against Adenovirus

Want to determine what antibody isotypes are directed against a specific antigen (in my case it was adenovirus).

 Wash buffer (10X):
 800 ml dH2O
 24.23 g Tris
 87.66 g NaCl
 3.73 g KCl
 5.0 ml Tween 20

1. Coat ELISA plate with 50 μg/mL Ad lysate (100 μL) overnight at 4°C

2. Wash 3x with wash buffer. Blot against paper towels.

3.