Sean Lauber:Adenovirus infection (in vitro)

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Possible to go from 20-50 pfu as well, although 10 seems to work just fine.
Possible to go from 20-50 pfu as well, although 10 seems to work just fine.
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PFU = MOI * #cells

Revision as of 11:59, 25 January 2013

This procedure is optimized for using 96-well plates (0.32 cm^2).

1. Seed 2000 cells per well

2. Incubate overnight at 37°C/5% CO2.

3. Wash cells with 100 ul of PBS two times.

4. Add 10 pfu/cell (MOI) of the virus in 10 ul of PBS on top of the cells.

5. Incubate at 37°C for 30 minutes.

6. Add 100 ul of media on top of the cells (don't remove the virus)

7. Incubate for as long as you need at 37°C/5% CO2


Possible to go from 20-50 pfu as well, although 10 seems to work just fine.


PFU = MOI * #cells

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