Sean Lauber:Adenovirus infection (in vitro)

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(New page: This procedure is optimized for using 96-well plates (0.32 cm^2). 1. Seed 2000 cells per well 2. Incubate overnight at 37°C/5% CO2. 3. Wash cells with 100 ul of PBS two times. 4. Add ...)
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7. Incubate for as long as you need at 37°C/5% CO2
7. Incubate for as long as you need at 37°C/5% CO2
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Possible to go from 20-50 pfu as well, although 10 seems to work just fine.

Revision as of 11:14, 11 December 2012

This procedure is optimized for using 96-well plates (0.32 cm^2).

1. Seed 2000 cells per well

2. Incubate overnight at 37°C/5% CO2.

3. Wash cells with 100 ul of PBS two times.

4. Add 10 pfu of the virus in 10 ul of PBS on top of the cells.

5. Incubate at 37°C for 30 minutes.

6. Add 100 ul of media on top of the cells (don't remove the virus)

7. Incubate for as long as you need at 37°C/5% CO2


Possible to go from 20-50 pfu as well, although 10 seems to work just fine.

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