Sean Clarke/109 notes: Difference between revisions
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6407 bp single-stranded circular DNA | 6407 bp single-stranded circular DNA | ||
filamentous, male-specific? coliphage | filamentous, male-specific? coliphage related to fd and f1 | ||
*p3,p8,p9 = virion structural proteins, p3 and p8 have N-term signal peptides that are hydrophobic | |||
9? coding genes | 9+? coding genes | ||
{| border="on" | {| border="on" | ||
! !! position !! notes !! size (aa) !! MW | ! !! position !! notes !! size (aa) !! MW | ||
|- | |- | ||
! | ! III | ||
| 1579-2853(1?) || capsid protein A, precursor has 18 extra aa, coding seq starts at 1633 || 424/408 || 42675 | | 1579-2853(1?) || capsid protein A, precursor has 18 extra aa, coding seq starts at 1633 || 424/408 || 42675 | ||
|- | |- | ||
! | ! VIII | ||
| 1301-1525 || encodes precursor w/23 extra N-term aa, pVIII is major virion capsid protein B | | 1301-1525 || encodes precursor w/23 extra N-term aa, pVIII is major virion capsid protein B | ||
|- | |- | ||
! | ! IX | ||
| 1206-1304(2?) || overlaps g8 by 1 nt, small virion capsid protein (C-protein) || 32 | | 1206-1304(2?) || overlaps g8 by 1 nt, small virion capsid protein (C-protein) || 32 | ||
|- | |- | ||
! | ! VI | ||
| 2856-3194 || small hydrophobic part of the capsid || 112 || 12264 | | 2856-3194 || small hydrophobic part of the capsid || 112 || 12264 | ||
|- | |- | ||
! | ! V | ||
| 843-1106 || helix-destabilizing protein in ss viral DNA synthesis | | 843-1106 || helix-destabilizing protein in ss viral DNA synthesis | ||
|- | |- | ||
! | ! II | ||
| 6006-831 || req'd for dsDNA replication || 410 || 46,117 | | 6006-831 || req'd for dsDNA replication || 410 || 46,117 | ||
|- | |- | ||
! | ! X | ||
| 496-831 || in frame with and initiated within g2 || || 12,670 | | 496-831 || in frame with and initiated within g2 || || 12,670 | ||
|- | |- | ||
! | ! VII | ||
| 1108-1209 || probably involved in phage assembly || 33 | | 1108-1209 || probably involved in phage assembly || 33 | ||
|- | |- | ||
! | ! I | ||
| 3196-4242 || probably phage assembly || 348 || 35000 in vitro, 39500 predicted | | 3196-4242 || probably phage assembly || 348 || 35000 in vitro, 39500 predicted | ||
|- | |- | ||
! | ! IV | ||
| 4220-5500 || phage assembly?, 23 nt overlap with C-term of gI, RBS 4204-27 || 426 || 45791 | | 4220-5500 || phage assembly?, 23 nt overlap with C-term of gI, RBS 4204-27 || 426 || 45791 | ||
|} | |} | ||
Revision as of 16:51, 15 January 2007
20.109(S07):_Genome_engineering_essay
M13 genome
6407 bp single-stranded circular DNA
filamentous, male-specific? coliphage related to fd and f1
- p3,p8,p9 = virion structural proteins, p3 and p8 have N-term signal peptides that are hydrophobic
9+? coding genes
| position | notes | size (aa) | MW | |
|---|---|---|---|---|
| III | 1579-2853(1?) | capsid protein A, precursor has 18 extra aa, coding seq starts at 1633 | 424/408 | 42675 |
| VIII | 1301-1525 | encodes precursor w/23 extra N-term aa, pVIII is major virion capsid protein B | ||
| IX | 1206-1304(2?) | overlaps g8 by 1 nt, small virion capsid protein (C-protein) | 32 | |
| VI | 2856-3194 | small hydrophobic part of the capsid | 112 | 12264 |
| V | 843-1106 | helix-destabilizing protein in ss viral DNA synthesis | ||
| II | 6006-831 | req'd for dsDNA replication | 410 | 46,117 |
| X | 496-831 | in frame with and initiated within g2 | 12,670 | |
| VII | 1108-1209 | probably involved in phage assembly | 33 | |
| I | 3196-4242 | probably phage assembly | 348 | 35000 in vitro, 39500 predicted |
| IV | 4220-5500 | phage assembly?, 23 nt overlap with C-term of gI, RBS 4204-27 | 426 | 45791 |
