Sbb14-Chris Anderson

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==[[User:JCAnderson|JCAnderson]] 13:05, 6 February 2014 (EST)==
==[[User:JCAnderson|JCAnderson]] 13:05, 6 February 2014 (EST)==
On tuesday, we did golden gate reactions.  We used the 5 plasmids that assemble into a cole1/KanR/GFP plasmid.  Those plasmids were obtained from Michael Lee in the Dueber lab.  The reactions were set up individually by each student according to the protocol on this wiki.  All but 3 or so of the reactions successfully gave green colonies.
On tuesday, we did golden gate reactions.  We used the 5 plasmids that assemble into a cole1/KanR/GFP plasmid.  Those plasmids were obtained from Michael Lee in the Dueber lab.  The reactions were set up individually by each student according to the protocol on this wiki.  All but 3 or so of the reactions successfully gave green colonies.

Revision as of 14:05, 6 February 2014

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JCAnderson 13:05, 6 February 2014 (EST)

On tuesday, we did golden gate reactions. We used the 5 plasmids that assemble into a cole1/KanR/GFP plasmid. Those plasmids were obtained from Michael Lee in the Dueber lab. The reactions were set up individually by each student according to the protocol on this wiki. All but 3 or so of the reactions successfully gave green colonies.

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