Sauer:plasmid vectors: Difference between revisions

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[[pBAD24]]
[[pBAD24]]
*ampicillin resistant; pBAD promoter
*ampicillin resistant; pBAD promoter
*we also have a version of this that Sean made called pBAD24N in which the NcoI site is changed to NdeI


[[pET21b]]
[[pET21b]]

Latest revision as of 10:25, 22 January 2006

SAUER LAB

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Here are the sequences to some plasmid vectors we commonly use:

pTrc99A

  • ampicillin resistant; pTrc promoter

pBAD24

  • ampicillin resistant; pBAD promoter

pET21b

  • ampicillin resistant; T7 promoter; has encoded C-terminal His6 tag

pET14b

  • ampicillin resistant; T7 promoter; has encoded N-terminal His6 tag

pET24d

  • kanamycin resistant; T7 promoter
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