Sauer:Silver staining

Who to ask about this protocol

Eyal, Kathleen

The recommended volume of solutions for mini-gels is 50 ml

- 1hr (or more) 50% EtOH, 12%HOAc, 0.5 ml/l Formaldehyde (37%)

- 2 x 20min 50% EtOH

- 20 sec ddw

- 1 min 0.2 gr/l Na2S2O3.5H2O

- 3 x 20 sec ddw

- 20 min 2 gr/l AgNO3, 750 mcl/l Formaldehyde (37%)

- 2 x 20 sec ddw

For developing prepare solutions in advance!

- 30 gr/l K2CO3, 500 mcl/l Formaldehyde (37%), smallest granule of Na2S2O3.5H2O you can find

- Shake gently untill developed (typically less than a minute)

- 1 min 1% Glycine

- 1 min ddw

- 10 min 1% Glycine

- 30 min ddw

This is an example of how it should look:

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