Sauer:Silver staining: Difference between revisions
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The recommended volume of solutions for mini-gels is 50 ml | The recommended volume of solutions for mini-gels is 50 ml | ||
- 1hr (or more) 50% EtOH, 12%HOAc, 0.5 ml/l Formaldehyde (37%) | - 1hr (or more) 50% EtOH, 12%HOAc, 0.5 ml/l Formaldehyde (37%) | ||
- 2 x 20min 50% EtOH | - 2 x 20min 50% EtOH | ||
- 20 sec ddw | - 20 sec ddw | ||
- 1 min 0.2 gr/l Na2S2O3.5H2O | - 1 min 0.2 gr/l Na2S2O3.5H2O | ||
- 3 x 20 sec ddw | - 3 x 20 sec ddw | ||
- 20 min 2 gr/l AgNO3, 750 mcl/l Formaldehyde (37%) | - 20 min 2 gr/l AgNO3, 750 mcl/l Formaldehyde (37%) | ||
- 2 x 20 sec ddw | - 2 x 20 sec ddw | ||
For developing prepare solutions in advance! | For developing prepare solutions in advance! | ||
- 30 gr/l K2CO3, 500 mcl/l Formaldehyde (37%), smallest granule of Na2S2O3.5H2O you can find | - 30 gr/l K2CO3, 500 mcl/l Formaldehyde (37%), smallest granule of Na2S2O3.5H2O you can find | ||
- Shake gently untill developed (typically less than a minute) | - Shake gently untill developed (typically less than a minute) | ||
- 1 min 1% Glycine | - 1 min 1% Glycine | ||
- 1 min ddw | - 1 min ddw | ||
- 10 min 1% Glycine | - 10 min 1% Glycine | ||
- 30 min ddw | - 30 min ddw | ||
[[Sauer Lab | Back to the Sauer lab]] | [[Sauer Lab | Back to the Sauer lab]] | ||
Revision as of 07:20, 11 August 2005
Who to ask about this protocol
Eyal, Kathleen
The recommended volume of solutions for mini-gels is 50 ml
- 1hr (or more) 50% EtOH, 12%HOAc, 0.5 ml/l Formaldehyde (37%)
- 2 x 20min 50% EtOH
- 20 sec ddw
- 1 min 0.2 gr/l Na2S2O3.5H2O
- 3 x 20 sec ddw
- 20 min 2 gr/l AgNO3, 750 mcl/l Formaldehyde (37%)
- 2 x 20 sec ddw
For developing prepare solutions in advance!
- 30 gr/l K2CO3, 500 mcl/l Formaldehyde (37%), smallest granule of Na2S2O3.5H2O you can find
- Shake gently untill developed (typically less than a minute)
- 1 min 1% Glycine
- 1 min ddw
- 10 min 1% Glycine
- 30 min ddw
