Sauer:Silver staining: Difference between revisions
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==Who to ask about this protocol== | ==Who to ask about this protocol== | ||
Eyal, Kathleen | Eyal, Kathleen | ||
The recommended volume of solutions for mini-gels is 50 ml | |||
- 1hr (or more) 50% EtOH, 12%HOAc, 0.5 ml/l Formaldehyde (37%) | |||
- 2 x 20min 50% EtOH | |||
- 20 sec ddw | |||
- 1 min 0.2 gr/l Na2S2O3.5H2O | |||
- 3 x 20 sec ddw | |||
- 20 min 2 gr/l AgNO3, 750 mcl/l Formaldehyde (37%) | |||
- 2 x 20 sec ddw | |||
For developing prepare solutions in advance! | |||
- 30 gr/l K2CO3, 500 mcl/l Formaldehyde (37%), smallest granule of Na2S2O3.5H2O you can find | |||
- Shake gently untill developed (typically less than a minute) | |||
- 1 min 1% Glycine | |||
- 1 min ddw | |||
- 10 min 1% Glycine | |||
- 30 min ddw | |||
[[Sauer Lab | Back to the Sauer lab]] | [[Sauer Lab | Back to the Sauer lab]] | ||
Revision as of 07:19, 11 August 2005
Who to ask about this protocol
Eyal, Kathleen
The recommended volume of solutions for mini-gels is 50 ml
- 1hr (or more) 50% EtOH, 12%HOAc, 0.5 ml/l Formaldehyde (37%) - 2 x 20min 50% EtOH - 20 sec ddw - 1 min 0.2 gr/l Na2S2O3.5H2O - 3 x 20 sec ddw - 20 min 2 gr/l AgNO3, 750 mcl/l Formaldehyde (37%) - 2 x 20 sec ddw
For developing prepare solutions in advance!
- 30 gr/l K2CO3, 500 mcl/l Formaldehyde (37%), smallest granule of Na2S2O3.5H2O you can find - Shake gently untill developed (typically less than a minute) - 1 min 1% Glycine - 1 min ddw - 10 min 1% Glycine - 30 min ddw
