Sauer:Protocols

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SAUER LAB

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Here we will post protocols that people in the lab generally use. Many of these will be lab-specific protocols, but if you think the protocol you are posting is more generally applicable, please link it to the general Protocols page. Also, if you typically do a variation of the protocol that is posted, please list it on the same page as a separate, alternative protocol. If possible, also try to note why what you do is different.


PLEASE NOTE: Blindly following a protocol is not a good substitute for actually talking to someone who has done the experiment. When posting protocols, please also include the name(s) of people in the lab who will be good to talk to about the protocol.

Analysis of proteins or RNA in vivo

Sauer:Western blot PROTOCOL NEEDED!

Sauer:Quantitative Western blot PROTOCOL NEEDED!

Sauer:Northern blot PROTOCOL NEEDED!

Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED!

Sauer:beta-Galactosidase Assay (A better Miller)

More in vivo goodies

Sauer:Competent cell preparation PROTOCOL NEEDED!

Sauer:P1vir phage transduction

Other good stuff

Generation of genomic libraries


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