Sauer:Protocols: Difference between revisions
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[[Sauer:P1vir phage transduction]] | [[Sauer:P1vir phage transduction]] | ||
[[Sauer:Plasmid-born | [[Sauer:Plasmid-born λRed recombineering using dsDNA]] | ||
===Other good stuff=== | ===Other good stuff=== |
Revision as of 12:03, 3 December 2007
Please link protocols to the general Protocols page if appropriate.
Protein Expression/PurificationSauer:Expression/purification of 35S-Met proteins Sauer:Purification of His6-tagged proteins Sauer:Lysing E. coli with Lysozymes In vitro enzymatic assaysSauer:In vitro degradation of GFP-ssrA by ClpXP Sauer:In vitro degradation of GFP-ssrA by ClpAP Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED! Proteins: in vitroSauer:bis-Tris SDS-PAGE, the very best Nucleic acids: in vitroSauer:In vitro transcription with T7 RNA polymerase Sauer:Phenol/chloroform extraction Sauer:Site-directed mutagenesis Sauer:RNA Purification from E. coli
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Analysis of proteins or RNA in vivoSauer:Quantitative Western blot Sauer:Northern blot PROTOCOL NEEDED! Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED! Sauer:beta-Galactosidase Assay (A better Miller) More in vivo goodiesSauer:Competent cell preparation PROTOCOL NEEDED! Sauer:P1vir phage transduction Sauer:Plasmid-born λRed recombineering using dsDNA Other good stuff |