Sauer:Protocols: Difference between revisions
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==Protein Expression/Purification== | ===Protein Expression/Purification=== | ||
[[Sauer:ClpX purification]] | [[Sauer:ClpX purification]] | ||
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[[Sauer:Expression/purification of 35S-Met proteins]] | [[Sauer:Expression/purification of 35S-Met proteins]] | ||
==''In vitro'' enzymatic assays== | ===''In vitro'' enzymatic assays=== | ||
[[Sauer:In vitro degradation of GFP-ssrA by ClpXP]] | [[Sauer:In vitro degradation of GFP-ssrA by ClpXP]] | ||
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[[Sauer:ATPase assays]] PROTOCOL NEEDED! | [[Sauer:ATPase assays]] PROTOCOL NEEDED! | ||
==Proteins: ''in vitro''== | ===Proteins: ''in vitro''=== | ||
[[Sauer:Silver staining]] | [[Sauer:Silver staining]] | ||
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[[Sauer:bis-Tris SDS-PAGE, the very best]] | [[Sauer:bis-Tris SDS-PAGE, the very best]] | ||
==Nucleic acids: ''in vitro''== | ===Nucleic acids: ''in vitro''=== | ||
[[Sauer:In vitro transcription with T7 RNA polymerase]] | [[Sauer:In vitro transcription with T7 RNA polymerase]] | ||
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==Analysis of proteins or RNA ''in vivo''== | ===Analysis of proteins or RNA ''in vivo''=== | ||
[[Sauer:Western blot]] PROTOCOL NEEDED! | [[Sauer:Western blot]] PROTOCOL NEEDED! | ||
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[[beta-Galactosidase Assay (A better Miller)| Sauer:beta-Galactosidase Assay (A better Miller)]] | [[beta-Galactosidase Assay (A better Miller)| Sauer:beta-Galactosidase Assay (A better Miller)]] | ||
==More ''in vivo'' goodies== | ===More ''in vivo'' goodies=== | ||
[[Sauer:Competent cell preparation]] PROTOCOL NEEDED! | [[Sauer:Competent cell preparation]] PROTOCOL NEEDED! | ||
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[[Sauer:P1vir phage transduction]] | [[Sauer:P1vir phage transduction]] | ||
==Other good stuff== | ===Other good stuff=== | ||
[[Library Generation|Generation of genomic libraries]] | [[Library Generation|Generation of genomic libraries]] | ||
Revision as of 20:50, 13 January 2006
Here we will post protocols that people in the lab generally use. Many of these will be lab-specific protocols, but if you think the protocol you are posting is more generally applicable, please link it to the general Protocols page. Also, if you typically do a variation of the protocol that is posted, please list it on the same page as a separate, alternative protocol. If possible, also try to note why what you do is different.
PLEASE NOTE: Blindly following a protocol is not a good substitute for actually talking to someone who has done the experiment. When posting protocols, please also include the name(s) of people in the lab who will be good to talk to about the protocol.
Protein Expression/PurificationSauer:ClpP purification PROTOCOL NEEDED! Sauer:Expression/purification of 35S-Met proteins In vitro enzymatic assaysSauer:In vitro degradation of GFP-ssrA by ClpXP Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED! Sauer:ATPase assays PROTOCOL NEEDED! Proteins: in vitroSauer:bis-Tris SDS-PAGE, the very best Nucleic acids: in vitroSauer:In vitro transcription with T7 RNA polymerase Sauer:Phenol/chloroform extraction Sauer:Nucleic acid quantitation Sauer:Site-directed mutagenesis
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Analysis of proteins or RNA in vivoSauer:Western blot PROTOCOL NEEDED! Sauer:Quantitative Western blot PROTOCOL NEEDED! Sauer:Northern blot PROTOCOL NEEDED! Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED! Sauer:beta-Galactosidase Assay (A better Miller) More in vivo goodiesSauer:Competent cell preparation PROTOCOL NEEDED! Sauer:P1vir phage transduction Other good stuff |
