Sauer:Protocols
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(→Nucleic acids: ''in vitro'') |
Current revision (17:34, 27 July 2009) (view source) (→Protein Expression/Purification) |
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[[Sauer:ClpX purification]] | [[Sauer:ClpX purification]] | ||
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| + | [[Sauer:His6 ClpX purification]] | ||
[[Sauer:ClpP purification]] | [[Sauer:ClpP purification]] | ||
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| + | [[Sauer:ClpA purification]] | ||
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| + | [[Sauer:SspB purification]] | ||
[[Sauer:Expression/purification of 35S-Met proteins]] | [[Sauer:Expression/purification of 35S-Met proteins]] | ||
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[[Sauer:Lysing E. coli with Lysozymes]] | [[Sauer:Lysing E. coli with Lysozymes]] | ||
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| + | [[Sauer:GFP-H6-ssrA purification]] | ||
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| + | [[Sauer:GST-sFtsH purification]] | ||
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| + | [[Sauer:FtsH purification]] | ||
===''In vitro'' enzymatic assays=== | ===''In vitro'' enzymatic assays=== | ||
[[Sauer:In vitro degradation of GFP-ssrA by ClpXP]] | [[Sauer:In vitro degradation of GFP-ssrA by ClpXP]] | ||
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| + | [[Sauer:In vitro degradation of GFP-ssrA by ClpAP]] | ||
[[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED! | [[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED! | ||
| - | [[Sauer:ATPase assays]] | + | [[Sauer:ATPase assays]] |
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| + | [[Sauer:In vitro peptide degradation by ClpXP]] | ||
===Proteins: ''in vitro''=== | ===Proteins: ''in vitro''=== | ||
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[[Sauer:RNA Purification from E. coli]] | [[Sauer:RNA Purification from E. coli]] | ||
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| + | [[Sauer:Stitching Genes by PCR]] | ||
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[[Sauer:P1vir phage transduction]] | [[Sauer:P1vir phage transduction]] | ||
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| + | [[Sauer:Plasmid-born λRed recombineering using dsDNA]] | ||
===Other good stuff=== | ===Other good stuff=== | ||
[[Library Generation|Generation of genomic libraries]] | [[Library Generation|Generation of genomic libraries]] | ||
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| + | [[Sauer:HPLC protocol]] | ||
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| + | [[Sauer:FPLC protocol]] | ||
</div> | </div> | ||
Current revision
Please link protocols to the general Protocols page if appropriate.
