Sauer:Protocols

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(Protein Expression/Purification)
Current revision (17:34, 27 July 2009) (view source)
(Protein Expression/Purification)
 
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[[Sauer:ClpX purification]]
[[Sauer:ClpX purification]]
 +
 +
[[Sauer:His6 ClpX purification]]
[[Sauer:ClpP purification]]  
[[Sauer:ClpP purification]]  
 +
 +
[[Sauer:ClpA purification]]
 +
 +
[[Sauer:SspB purification]]
[[Sauer:Expression/purification of 35S-Met proteins]]
[[Sauer:Expression/purification of 35S-Met proteins]]
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[[Sauer:Lysing E. coli with Lysozymes]]
[[Sauer:Lysing E. coli with Lysozymes]]
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 +
[[Sauer:GFP-H6-ssrA purification]]
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 +
[[Sauer:GST-sFtsH purification]]
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[[Sauer:FtsH purification]]
===''In vitro'' enzymatic assays===
===''In vitro'' enzymatic assays===
[[Sauer:In vitro degradation of GFP-ssrA by ClpXP]]
[[Sauer:In vitro degradation of GFP-ssrA by ClpXP]]
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[[Sauer:In vitro degradation of GFP-ssrA by ClpAP]]
[[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED!
[[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED!
-
[[Sauer:ATPase assays]]  
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[[Sauer:ATPase assays]]
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[[Sauer:In vitro peptide degradation by ClpXP]]
===Proteins: ''in vitro''===
===Proteins: ''in vitro''===
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[[Sauer:Phenol/chloroform extraction]]
[[Sauer:Phenol/chloroform extraction]]
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[[Sauer:Nucleic acid quantitation]]
+
[[Quantitating nucleic acids]]
[[Sauer:Site-directed mutagenesis]]
[[Sauer:Site-directed mutagenesis]]
[[Sauer:RNA Purification from E. coli]]
[[Sauer:RNA Purification from E. coli]]
 +
 +
[[Sauer:Stitching Genes by PCR]]
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===Analysis of proteins or RNA ''in vivo''===
===Analysis of proteins or RNA ''in vivo''===
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[[Sauer:Western blot]] PROTOCOL NEEDED!
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[[Sauer:Western blot]]
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[[Sauer:Quantitative Western blot]] PROTOCOL NEEDED!
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[[Sauer:Quantitative Western blot]]
[[Sauer:Northern blot]] PROTOCOL NEEDED!
[[Sauer:Northern blot]] PROTOCOL NEEDED!
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[[Sauer:P1vir phage transduction]]
[[Sauer:P1vir phage transduction]]
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 +
[[Sauer:Plasmid-born λRed recombineering using dsDNA]]
===Other good stuff===
===Other good stuff===
[[Library Generation|Generation of genomic libraries]]
[[Library Generation|Generation of genomic libraries]]
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 +
[[Sauer:HPLC protocol]]
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[[Sauer:FPLC protocol]]
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Current revision

SAUER LAB

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Please link protocols to the general Protocols page if appropriate.

Protein Expression/Purification

Sauer:ClpX purification

Sauer:His6 ClpX purification

Sauer:ClpP purification

Sauer:ClpA purification

Sauer:SspB purification

Sauer:Expression/purification of 35S-Met proteins

Sauer:Purification of His6-tagged proteins

Sauer:Lysing E. coli with Lysozymes

Sauer:GFP-H6-ssrA purification

Sauer:GST-sFtsH purification

Sauer:FtsH purification

In vitro enzymatic assays

Sauer:In vitro degradation of GFP-ssrA by ClpXP

Sauer:In vitro degradation of GFP-ssrA by ClpAP

Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED!

Sauer:ATPase assays

Sauer:In vitro peptide degradation by ClpXP

Proteins: in vitro

Sauer:Silver staining

Sauer:bis-Tris SDS-PAGE, the very best

Nucleic acids: in vitro

Sauer:In vitro transcription with T7 RNA polymerase

Sauer:Phenol/chloroform extraction

Quantitating nucleic acids

Sauer:Site-directed mutagenesis

Sauer:RNA Purification from E. coli

Sauer:Stitching Genes by PCR



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