Sauer:Protocols
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Current revision (17:34, 27 July 2009) (view source) (→Protein Expression/Purification) |
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{{Sauer lab sidebar}} | {{Sauer lab sidebar}} | ||
| - | + | Please link protocols to the general [[Protocols]] page if appropriate. | |
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| - | + | ===Protein Expression/Purification=== | |
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[[Sauer:ClpX purification]] | [[Sauer:ClpX purification]] | ||
| - | [[Sauer: | + | [[Sauer:His6 ClpX purification]] |
| - | [[Sauer: | + | [[Sauer:ClpP purification]] |
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| + | [[Sauer:ClpA purification]] | ||
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| + | [[Sauer:SspB purification]] | ||
[[Sauer:Expression/purification of 35S-Met proteins]] | [[Sauer:Expression/purification of 35S-Met proteins]] | ||
| - | [[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] | + | [[Sauer:Purification of His-tagged proteins|Sauer:Purification of His<sub>6</sub>-tagged proteins]] |
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| + | [[Sauer:Lysing E. coli with Lysozymes]] | ||
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| + | [[Sauer:GFP-H6-ssrA purification]] | ||
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| + | [[Sauer:GST-sFtsH purification]] | ||
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| + | [[Sauer:FtsH purification]] | ||
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| + | ===''In vitro'' enzymatic assays=== | ||
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| + | [[Sauer:In vitro degradation of GFP-ssrA by ClpXP]] | ||
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| + | [[Sauer:In vitro degradation of GFP-ssrA by ClpAP]] | ||
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| + | [[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED! | ||
[[Sauer:ATPase assays]] | [[Sauer:ATPase assays]] | ||
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| + | [[Sauer:In vitro peptide degradation by ClpXP]] | ||
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| + | ===Proteins: ''in vitro''=== | ||
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| + | [[Sauer:Silver staining]] | ||
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| + | [[Sauer:bis-Tris SDS-PAGE, the very best]] | ||
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| + | ===Nucleic acids: ''in vitro''=== | ||
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| + | [[Sauer:In vitro transcription with T7 RNA polymerase]] | ||
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| + | [[Sauer:Phenol/chloroform extraction]] | ||
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| + | [[Quantitating nucleic acids]] | ||
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| + | [[Sauer:Site-directed mutagenesis]] | ||
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| + | [[Sauer:RNA Purification from E. coli]] | ||
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| + | [[Sauer:Stitching Genes by PCR]] | ||
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| + | </div> | ||
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| + | <div style="clear: right; text-align: left; float: left; padding: .4em .9em .9em"> | ||
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| + | ===Analysis of proteins or RNA ''in vivo''=== | ||
[[Sauer:Western blot]] | [[Sauer:Western blot]] | ||
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[[Sauer:Quantitative Western blot]] | [[Sauer:Quantitative Western blot]] | ||
| - | [[Sauer: | + | [[Sauer:Northern blot]] PROTOCOL NEEDED! |
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| + | [[Sauer:In vivo tmRNA tagging assays]] PROTOCOL NEEDED! | ||
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| + | [[beta-Galactosidase Assay (A better Miller)| Sauer:beta-Galactosidase Assay (A better Miller)]] | ||
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| + | ===More ''in vivo'' goodies=== | ||
| - | [[Sauer:Competent cell preparation]] | + | [[Sauer:Competent cell preparation]] PROTOCOL NEEDED! |
[[Sauer:P1vir phage transduction]] | [[Sauer:P1vir phage transduction]] | ||
| - | [[Sauer: | + | [[Sauer:Plasmid-born λRed recombineering using dsDNA]] |
| - | [[ | + | ===Other good stuff=== |
| + | [[Library Generation|Generation of genomic libraries]] | ||
| - | [[Sauer: | + | [[Sauer:HPLC protocol]] |
| - | [[Sauer: | + | [[Sauer:FPLC protocol]] |
| - | + | </div> | |
| + | |} | ||
| - | + | __NOTOC__ | |
Current revision
Please link protocols to the general Protocols page if appropriate.
