Sauer:Protocols: Difference between revisions

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[[Sauer:ClpX purification]]
[[Sauer:ClpX purification]]
[[Sauer:His6 ClpX purification]]


[[Sauer:ClpP purification]]  
[[Sauer:ClpP purification]]  
[[Sauer:ClpA purification]]


[[Sauer:SspB purification]]
[[Sauer:SspB purification]]
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[[Sauer:Lysing E. coli with Lysozymes]]
[[Sauer:Lysing E. coli with Lysozymes]]
[[Sauer:GFP-H6-ssrA purification]]
[[Sauer:GST-sFtsH purification]]
[[Sauer:FtsH purification]]


===''In vitro'' enzymatic assays===
===''In vitro'' enzymatic assays===
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[[Sauer:ATPase assays]]
[[Sauer:ATPase assays]]
[[Sauer:In vitro peptide degradation by ClpXP]]


===Proteins: ''in vitro''===
===Proteins: ''in vitro''===
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[[Sauer:P1vir phage transduction]]
[[Sauer:P1vir phage transduction]]
[[Sauer:Plasmid-born λRed recombineering using dsDNA]]


===Other good stuff===
===Other good stuff===
[[Library Generation|Generation of genomic libraries]]
[[Library Generation|Generation of genomic libraries]]
[[Sauer:HPLC protocol]]
[[Sauer:FPLC protocol]]


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Latest revision as of 14:34, 27 July 2009

SAUER LAB

Home        Protocols        Lab Members        Materials        Equipment        Links        Internal       

Please link protocols to the general Protocols page if appropriate.

Protein Expression/Purification

Sauer:ClpX purification

Sauer:His6 ClpX purification

Sauer:ClpP purification

Sauer:ClpA purification

Sauer:SspB purification

Sauer:Expression/purification of 35S-Met proteins

Sauer:Purification of His6-tagged proteins

Sauer:Lysing E. coli with Lysozymes

Sauer:GFP-H6-ssrA purification

Sauer:GST-sFtsH purification

Sauer:FtsH purification

In vitro enzymatic assays

Sauer:In vitro degradation of GFP-ssrA by ClpXP

Sauer:In vitro degradation of GFP-ssrA by ClpAP

Sauer:In vitro degradation of radiolabeled proteins by ClpXP PROTOCOL NEEDED!

Sauer:ATPase assays

Sauer:In vitro peptide degradation by ClpXP

Proteins: in vitro

Sauer:Silver staining

Sauer:bis-Tris SDS-PAGE, the very best

Nucleic acids: in vitro

Sauer:In vitro transcription with T7 RNA polymerase

Sauer:Phenol/chloroform extraction

Quantitating nucleic acids

Sauer:Site-directed mutagenesis

Sauer:RNA Purification from E. coli

Sauer:Stitching Genes by PCR