Sauer:Protocols

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[[Sauer:ClpX purification]]
[[Sauer:ClpX purification]]
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[[Sauer:ClpP purification]] NEEDS TO BE FILLED IN, WORK ON ME!
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[[Sauer:ClpP purification]] PROTOCOL NEEDED!
[[Sauer:Expression/purification of 35S-Met proteins]]
[[Sauer:Expression/purification of 35S-Met proteins]]
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[[Sauer:In vitro degradation of GFP-ssrA by ClpXP]]
[[Sauer:In vitro degradation of GFP-ssrA by ClpXP]]
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[[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] NEEDS TO BE FILLED IN, WORK ON ME!
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[[Sauer:In vitro degradation of radiolabeled proteins by ClpXP]] PROTOCOL NEEDED!
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[[Sauer:ATPase assays]] NEEDS TO BE FILLED IN, WORK ON ME!
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[[Sauer:ATPase assays]] PROTOCOL NEEDED!
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==Analysis of proteins or RNA ''in vivo''==
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[[Sauer:Western blot]] NEEDS TO BE FILLED IN, WORK ON ME!
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[[Sauer:Quantitative Western blot]] NEEDS TO BE FILLED IN, WORK ON ME!
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[[Sauer:Northern blot]] NEEDS TO BE FILLED IN, WORK ON ME!
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[[Sauer:In vivo tmRNA tagging assays]] NEEDS TO BE FILLED IN, WORK ON ME!
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[[beta-Galactosidase Assay (A better Miller)| Sauer:beta-Galactosidase Assay (A better Miller)]]
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==Proteins: ''in vitro''==
==Proteins: ''in vitro''==
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==Analysis of proteins or RNA ''in vivo''==
 +
 
 +
[[Sauer:Western blot]] PROTOCOL NEEDED!
 +
 
 +
[[Sauer:Quantitative Western blot]] PROTOCOL NEEDED!
 +
 
 +
[[Sauer:Northern blot]] PROTOCOL NEEDED!
 +
 
 +
[[Sauer:In vivo tmRNA tagging assays]] PROTOCOL NEEDED!
 +
 
 +
[[beta-Galactosidase Assay (A better Miller)| Sauer:beta-Galactosidase Assay (A better Miller)]]
==More ''in vivo'' goodies==
==More ''in vivo'' goodies==
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[[Sauer:Competent cell preparation]] NEEDS TO BE FILLED IN, WORK ON ME!
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[[Sauer:Competent cell preparation]] PROTOCOL NEEDED!
[[Sauer:P1vir phage transduction]]
[[Sauer:P1vir phage transduction]]

Revision as of 22:47, 13 January 2006

SAUER LAB

Home        Protocols        Lab Members        Materials        Equipment        Links        Internal       

Here we will post protocols that people in the lab generally use. Many of these will be lab-specific protocols, but if you think the protocol you are posting is more generally applicable, please link it to the general Protocols page. Also, if you typically do a variation of the protocol that is posted, please list it on the same page as a separate, alternative protocol. If possible, also try to note why what you do is different.


PLEASE NOTE: Blindly following a protocol is not a good substitute for actually talking to someone who has done the experiment. When posting protocols, please also include the name(s) of people in the lab who will be good to talk to about the protocol.

Analysis of proteins or RNA in vivo

Sauer:Western blot PROTOCOL NEEDED!

Sauer:Quantitative Western blot PROTOCOL NEEDED!

Sauer:Northern blot PROTOCOL NEEDED!

Sauer:In vivo tmRNA tagging assays PROTOCOL NEEDED!

Sauer:beta-Galactosidase Assay (A better Miller)

More in vivo goodies

Sauer:Competent cell preparation PROTOCOL NEEDED!

Sauer:P1vir phage transduction

Other good stuff

Generation of genomic libraries


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