Sauer:Expression/purification of 35S-Met proteins

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
Line 1: Line 1:
 +
{{Sauer lab sidebar}}
*In general, the Sauer lab only radiolabels His<sub>6</sub>-tagged proteins because they are easy to purify without getting radiation all over the place. If necessary, however, other methods of purification can be employed, but it is recommended that gravity columns be used versus an FPLC-type system in order to reduce the spread of radiation.
*In general, the Sauer lab only radiolabels His<sub>6</sub>-tagged proteins because they are easy to purify without getting radiation all over the place. If necessary, however, other methods of purification can be employed, but it is recommended that gravity columns be used versus an FPLC-type system in order to reduce the spread of radiation.
*In general, either of these protocols should work for your protein of choice. Major differences include the time of induction with and without radiolabel, and native versus denaturing protein purification by nickel column affinity.
*In general, either of these protocols should work for your protein of choice. Major differences include the time of induction with and without radiolabel, and native versus denaturing protein purification by nickel column affinity.
Line 10: Line 11:
==Who to ask about this protocol==
==Who to ask about this protocol==
[[user:kathmc|Kathleen]], Andreas
[[user:kathmc|Kathleen]], Andreas
-
 
-
[[Sauer Lab | Back to the Sauer lab]]
 

Revision as of 21:23, 20 September 2005

SAUER LAB

Home        Protocols        Lab Members        Materials        Equipment        Links        Internal       

  • In general, the Sauer lab only radiolabels His6-tagged proteins because they are easy to purify without getting radiation all over the place. If necessary, however, other methods of purification can be employed, but it is recommended that gravity columns be used versus an FPLC-type system in order to reduce the spread of radiation.
  • In general, either of these protocols should work for your protein of choice. Major differences include the time of induction with and without radiolabel, and native versus denaturing protein purification by nickel column affinity.

I27 expression/purification

Arc expression/purification


Who to ask about this protocol

Kathleen, Andreas

Personal tools