Sauer:Expression/purification of 35S-Met proteins
From OpenWetWare
(Difference between revisions)
Current revision (13:43, 9 May 2007) (view source) |
|||
| (11 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | + | {{Sauer lab sidebar}} | |
| + | *In general, the Sauer lab only radiolabels His<sub>6</sub>-tagged proteins because they are easy to purify without getting radiation all over the place. If necessary, however, other methods of purification can be employed, but it is recommended that gravity columns be used versus an FPLC-type system in order to reduce the spread of radiation. | ||
| + | |||
| + | [[Sauer:Expression/purification of 35S-Met proteins/Native purification]] | ||
| + | |||
| + | [[Category:Protocol]] | ||
| + | [[Category:In vitro]] | ||
| + | [[Category:Protein]] | ||
Current revision
- In general, the Sauer lab only radiolabels His6-tagged proteins because they are easy to purify without getting radiation all over the place. If necessary, however, other methods of purification can be employed, but it is recommended that gravity columns be used versus an FPLC-type system in order to reduce the spread of radiation.
Sauer:Expression/purification of 35S-Met proteins/Native purification
