Sauer:Davis Minimal Media
From OpenWetWare
Davis Minimal Media (10x) 1 L:
K2HPO4 - 90 gr
KH2PO4 - 30 gr
(NH4)SO4 - 20 gr
NaCitrate - 5 gr
MgSO4100x (100gr/L) (1%)
Glucose100x 20%
vitamin B1 (thiamine)2000x 2 mg/mL
Amino Acids200x 10 mg/mL
Plates (1 L)
800 mL H20 ddw + 20 gr agar --> autoclave (in hebrew)
Davis10x 100 mL
Glucose 10 mL
1% MgSO4 10 mL
Antibiotic weighted
DDW 80 mL Sterile
