Saponin Lysis of RBCs: Difference between revisions
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Lyse red blood cells while leaving the ''Plasmodium falciparum'' parasite intact with it's parasite membrane and parasitophorous vacoule membrane. Typically used right before freezing down parasites for genomic DNA extraction, or for getting rid of hemoglobin right before running a Western Blot on parasite extracts. | Lyse red blood cells while leaving the ''Plasmodium falciparum'' parasite intact with it's parasite membrane and parasitophorous vacoule membrane. Typically used right before freezing down parasites for genomic DNA extraction, or for getting rid of hemoglobin right before running a Western Blot on parasite extracts. | ||
===Reagents=== | ===Reagents=== | ||
*0.15% Saponin in PBS | |||
*1X PBS | |||
*12 mL of Plasmodium falciparum blood culture at 4% hematocrit | |||
===Equipment=== | ===Equipment=== | ||
*Refrigerated microcentrifuge | |||
==Procedure== | ==Procedure== | ||
Revision as of 19:38, 28 September 2009
Curators
Jingyang Chen, Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle, WA, 98109, USA. jingyang.chen@sbri.org
Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is currently discussing the idea of protocol curators. Please contribute.
Abstract
Lyse red blood cells while leaving the Plasmodium falciparum parasite intact with it's parasite membrane and parasitophorous vacoule membrane. Typically used right before freezing down parasites for genomic DNA extraction, or for getting rid of hemoglobin right before running a Western Blot on parasite extracts.
Reagents
- 0.15% Saponin in PBS
- 1X PBS
- 12 mL of Plasmodium falciparum blood culture at 4% hematocrit
Equipment
- Refrigerated microcentrifuge
Procedure
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Critical steps
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Notes
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References
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Specific Protocols
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