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[[Image:sackshot.jpg|thumb|right]]
__TOC__
Jon Sack, Ph.D.


==Research interests==
Electrical signaling in living cells controls a wide variety of arguably important physiological processes such as feeling, thinking, and heartbeat. Electrophysiological signals are created by proteins known as ion channels, and modulating the behavior of ion channels will alter the processes they control.  The goal of my research program is to develop modulators selective for ion channel subtypes, to more precisely alter electrophysiological signals and identify channel subunits that generate native currents.  Establishing the molecular identity of voltage-gated potassium (Kv) channels has been a particularly challenging problem: mammalian channels arise from a family of more than 40 genes, and pore-forming subunits can assemble as heterotetramers. Despite substantial and enduring efforts, few modulators of Kv channel activity have been discovered that are highly selective between  channel subtypes.  This is perhaps due to a high degree of sequence conservation between subfamily members in functionally important transmembrane segments. Our research efforts seek to enhance the selectivity of channel modulators by covalent attachment to benign, yet well-targeted, biologics.


==Education==
----
<!--Include info about your educational background-->
* Ph.D., Stanford University, Department of Biological Sciences
* B.A., Reed College, Biochemistry


==Institutional Affiliation==
== My Test of OpenWetWare Editing ==
I would like to try the following editing tasks:
* Add a [hyperlink](https://uk.yahoo.com/?ilc=400).
** For example: Link to the paper can be found HERE[http://www.nature.com/nature/journal/v403/n6767/pdf/403339a0.pdf].
* Add a document or Dropbox link:
** Addition of document: [[Image:Example.jpg]]
** Addition of Dropbox link: The file can be downloaded from Dropbox via this [LINK]
* Cite a paper.
** Hopefully see the list of references at the bottom of the page.
** This is a really interesting study <ref>Reference goes here</ref>
* Add a picture.


Assistant Professor
== References ==
{{Reflist}}


Department of Physiology & Membrane Biology
----


School of Medicine
'''EmbryoName HK023- chicken embryo- Stage 19.5 - 8.12.14'''


University of California


4126 Tupper Hall
==Experiment==


One Shields Avenue
'''Objective'''
To transform HB101s with a plasmid encoding GFP (called pGFP) and express this protein by inducing with arabinose. Testing out openwetware
'''Procedure'''


Davis, California 95616
* Trigeminal nerve bilaterally DiO crystals
* 7th/8th nerve bilaterally DiI crystals (Big chunk on 7th nerve on L side and big chunk on 8th on R side)


530.752.4131 tel
'''Notes'''
530.752.5314 lab tel
No obvious contamination, purposelly put a greater chunk of dye on the 7th nerve on the L side and the 8th nerve on the R side so will see if there is any visible difference between the amount of neurons labelled in different positions within the rhombomere.  
530.752.5423 fax


jsack@ucdavis.edu
==Files==


==Math Practice==
*
*
*
*


''x''<sup>2</sup>+''y''<sup>2</sup>=''z''<sup>2</sup>
*
** abd dfg
**


{{math|''x''<sup>2</sup>+''y''<sup>2</sup>{{=}}''z''<sup>2</sup>}}
==Results==


<math>x^2+y^2=z^2</math>
===Facial===
(Green)


&sum;i=∞
* '''''Afferents'''''
 


<math>\frac{\sqrt{2\alpha_1+e^2}}{\log \alpha_2}</math>
* '''''Efferents'''''


==Google Docs==
 
{{#widget:Google Document
 
|id=19hEjXbw_pwVjY5vwu-hQGfqpLTuwWQemRzEsCvJw640
 
}}
 
 
===VIII ===
(green)
 
* '''''Afferents'''''
 
 
 
* '''''Efferents'''''
 
 
* '''''Other'''''
 
 
 
===Basal plate ===
(red)
 
 
==Summary==
 
'''Questions'''
 
'''Future'''
 
 
<META HTTP-EQUIV="refresh" CONTENT="10;URL=http://my.newplace.com/">
 
 
Mary {pad|4em} had a little lamb.
 
'''Badrul Arefin'''
 
 
# Jeong M^, {{spaces|7}} <u>Sun D^</u>, Luo M^, Huang Y, Challen GA, <u>Rodriguez B</u>, Zhang X, Chavez L, Wang H, Hannah R, Kim S, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, <u>Li W*</u>, Goodell MA*: Large conserved domains of low DNA methylation maintained by Dnmt3a. <br/> '''''          Nature Genetics''''' . Accepted.
# <u>Akdemir KC</u>, Jain AK, Alton K, Arronow B, J. CA, ..., <u>Li W*</u>, Barton MC*: Genome Wide Profiling Reveals Stimulus-specific Functions of p53 During Differentiation and DNA Damage of Human Embryonic Stem Cells. <br/>'''''Nucleic Acids Res.''''' Accepted
# Klein JB, Piao L, …, <u>Li W</u>, Shi X, Kutateladze TG: KDM5B demethylase binds to its histone target and the product through distinct modules.<br/>''Cell Reports''. Accepted. <br/><br/>'''2013''' <br/><br/>
# <u>Chen K</u>, <u>Xi Y</u>, Pan X, Dent S, He X, <u>Li W*</u>: DANPOS: Dynamic Analysis of Nucleosome Position and Occupancy by Sequencing. <br/>'''''Genome Res.''''' 23 (2013) 341-351. [http://www.bcm.edu/news/item.cfm?newsID=6723 BCM News];  [http://epigenie.com/danpos-reveals-dynamic-nucleosomes Epigenie Headline]  <br/><br/>
# <u>Wang L</u>, <u>Park JH</u>, Wang S, Kocher JP, <u>Li W*</u>: CPAT: Coding-Potential Assessment Tool Using an Alignment-Free Logistic Regression Model. <br/>'''''Nucleic Acids Res.''''' 41 (2013) e74.<br/><br/>
# <u>Lin X</u>, <u>Sun D</u>, <u>Rodriguez B</u>, Zhao Q, Sun H, Zhang Y, <u>Li W*</u>: BseQC: Quality Control of bisulfite sequencing experiments. <br/>'''''Bioinformatics''''' 29 (2013) 3227-3229. PMCID: PMC3842756 .<br/><br/>
# <u>Chen K^</u>, Wilson MA^, Hirsch C, Watson A, Liang S, Lu Y, <u>Li W*</u>, Dent S*: Stabilization of the Promoter Nucleosomes in Nucleosome Free Region by the Yeast Ssn6-Tup1 Corepressor. <br/>'''''Genome Res.''''' 23 (2013) 312-322. <br/><br/>

Revision as of 21:58, 6 March 2016


My Test of OpenWetWare Editing

I would like to try the following editing tasks:

  • Add a [hyperlink](https://uk.yahoo.com/?ilc=400).
    • For example: Link to the paper can be found HERE[1].
  • Add a document or Dropbox link:
    • Addition of document:
    • Addition of Dropbox link: The file can be downloaded from Dropbox via this [LINK]
  • Cite a paper.
    • Hopefully see the list of references at the bottom of the page.
    • This is a really interesting study [1]
  • Add a picture.

References

  1. ↑ Reference goes here

EmbryoName HK023- chicken embryo- Stage 19.5 - 8.12.14


Experiment

Objective To transform HB101s with a plasmid encoding GFP (called pGFP) and express this protein by inducing with arabinose. Testing out openwetware Procedure

  • Trigeminal nerve bilaterally DiO crystals
  • 7th/8th nerve bilaterally DiI crystals (Big chunk on 7th nerve on L side and big chunk on 8th on R side)

Notes No obvious contamination, purposelly put a greater chunk of dye on the 7th nerve on the L side and the 8th nerve on the R side so will see if there is any visible difference between the amount of neurons labelled in different positions within the rhombomere.

Files

    • abd dfg

Results

Facial

(Green)

  • Afferents


  • Efferents




VIII

(green)

  • Afferents


  • Efferents


  • Other


Basal plate

(red)


Summary

Questions

Future


<META HTTP-EQUIV="refresh" CONTENT="10;URL=http://my.newplace.com/">


Mary {pad|4em} had a little lamb.

Badrul Arefin


  1. Jeong M^, Template:Spaces Sun D^, Luo M^, Huang Y, Challen GA, Rodriguez B, Zhang X, Chavez L, Wang H, Hannah R, Kim S, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, Li W*, Goodell MA*: Large conserved domains of low DNA methylation maintained by Dnmt3a.
    Nature Genetics . Accepted.
  2. Akdemir KC, Jain AK, Alton K, Arronow B, J. CA, ..., Li W*, Barton MC*: Genome Wide Profiling Reveals Stimulus-specific Functions of p53 During Differentiation and DNA Damage of Human Embryonic Stem Cells.
    Nucleic Acids Res. Accepted
  3. Klein JB, Piao L, …, Li W, Shi X, Kutateladze TG: KDM5B demethylase binds to its histone target and the product through distinct modules.
    Cell Reports. Accepted.

    2013

  4. Chen K, Xi Y, Pan X, Dent S, He X, Li W*: DANPOS: Dynamic Analysis of Nucleosome Position and Occupancy by Sequencing.
    Genome Res. 23 (2013) 341-351. BCM News; Epigenie Headline

  5. Wang L, Park JH, Wang S, Kocher JP, Li W*: CPAT: Coding-Potential Assessment Tool Using an Alignment-Free Logistic Regression Model.
    Nucleic Acids Res. 41 (2013) e74.

  6. Lin X, Sun D, Rodriguez B, Zhao Q, Sun H, Zhang Y, Li W*: BseQC: Quality Control of bisulfite sequencing experiments.
    Bioinformatics 29 (2013) 3227-3229. PMCID: PMC3842756 .

  7. Chen K^, Wilson MA^, Hirsch C, Watson A, Liang S, Lu Y, Li W*, Dent S*: Stabilization of the Promoter Nucleosomes in Nucleosome Free Region by the Yeast Ssn6-Tup1 Corepressor.
    Genome Res. 23 (2013) 312-322.

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