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<biblio>
#Frost pmid=7915817
#Jaenecke pmid=8858584
#Lawley pmid=12855161
#Lessl pmid=8407818
#Miller pmid=2993231
#Martinez-Morales pmid=10559184
#Wilkins pmid=12220405
</biblio>


<biblio>
# <nowiki></nowiki>pmid=7915817
</biblio>


<SUP><!-- ## START CALENDAR COLUMN -->
__TOC__
<LNCALENDAR></LNCALENDAR>
<!-- ## END CALENDAR COLUMN -->


==EXPANDING A TEMPLATE==
'''EmbryoName HK023- chicken embryo- Stage 19.5 - 8.12.14'''
BASED ON [[TEMPLATE:FILEDESCRIPTION]]:
<NOWIKI>
{{FILEDESCRIPTION |
NAME=NAME |
DATE=20081012 |
DESCRIPTION=HELLO I'M A DESCRIPTION |
COMMENT=HELLO I'M A COMMENT
}}</NOWIKI>
==RESULT==
{{FILEDESCRIPTION |NAME=NAME |DATE=20081012 |DESCRIPTION=HELLO I'M A DESCRIPTION |COMMENT=HELLO I'M A COMMENT}}


{| {{TABLE|WIDTH: 73PX}}
| ALIGN="CENTER" STYLE="BACKGROUND:#F0F0F0;"|'''THIS'''
| ALIGN="CENTER" STYLE="BACKGROUND:#F0F0F0;"|'''17'''
| ALIGN="CENTER" STYLE="BACKGROUND:#F0F0F0;"|'''3.44'''
|-
| IS||29||2.33
|-
| A||39||5.31
|-
| TEST||44||45.3
|-
|}


--[[USER:LINDENB|LINDENB]] 10:01, 8 DECEMBER 2008 (EST)
==Experiment==


VOLUNTEER: JULIE NORVILLE
'''Objective'''
DATE: (SIGNED UP) JANUARY 26, 2009-(EXPECTS TO BE ACTIVE TILL) 2010
To transform HB101s with a plasmid encoding GFP (called pGFP) and express this protein by inducing with arabinose. Testing out openwetware
BIO: A GRADUATE STUDENT AT MIT
'''Procedure'''
CONTACT: DIYOUTREACH AT GMAIL DOT COM
(IF YOU DO NOT WANT THIS LISTED, YOU MAY PUT CONFIDENTIAL-CONTACT DIYOUTREACH AND SEND YOUR CONTACT INFO TO
DIYOUTREACH AT GMAIL DOT COM)
INTERESTS: DEVELOPING CHEAP BIOLOGICAL TOOLS AND TEACHING MATERIALS
UNIVERSITY AFFILIATIONS: MIT (CURRENT), CALTECH (ALUMNA)
GEOGRAPHICAL LOCATION: BOSTON AREA


*'''[[USER:KENNY FREUNDLICH|KENNY FREUNDLICH]] 17:04, 6 APRIL 2009 (EDT)''':
* Trigeminal nerve bilaterally DiO crystals
* 7th/8th nerve bilaterally DiI crystals (Big chunk on 7th nerve on L side and big chunk on 8th on R side)


BELOW IS ONE IMAGE.
'''Notes'''
No obvious contamination, purposelly put a greater chunk of dye on the 7th nerve on the L side and the 8th nerve on the R side so will see if there is any visible difference between the amount of neurons labelled in different positions within the rhombomere.  


LINK TO [[BIOTEST]]
==Files==


[[IMAGE:WELLESLEY-QUAD.JPEG‎]]
*
*
*
*


[[IMAGE:ABSTRACT.PDF]]
*
**
**


==Results==


==TESTING WIKED==
===Facial===
(Green)


X<SUP>2</SUP>
* '''''Afferents'''''
 


<sub>sdfds
* '''''Efferents'''''


<script src="http://pubget.com/widgetizer/render_js?q=stem%20cell%20RNA-Seq&count=5"> </script> <noscript> <a href="http://pubget.com/search?q=stem%20cell%20RNA-Seq">stem cell RNA-Seq</a> </noscript>
 
</sub>
 
[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WWK-525RKWB-7-1&_cdi=7133&_user=1072900&_pii=S1535610811000420&_origin=gateway&_coverDate=02%2F15%2F2011&_sk=%23TOC%237133%232011%23999809997%232907735%23FLA%23display%23Volume_19,_Issue_2,_Pages_157-294_(15_February_2011)%23tagged%23Volume%23first%3D19%23Issue%23first%3D2%23date%23(15_February_2011)%23&view=c&_gw=y&wchp=dGLbVzW-zSkWb&md5=04be5f8d0326da5305d046357fbf4452&ie=/sdarticle.pdf MSC paper]
 
 
 
===VIII ===
(green)
 
* '''''Afferents'''''
 
 
 
* '''''Efferents'''''
 
 
* '''''Other'''''
 
 
 
===Basal plate ===
(red)
 
 
==Summary==
 
'''Questions'''
 
'''Future'''
 
 
<META HTTP-EQUIV="refresh" CONTENT="10;URL=http://my.newplace.com/">
 
 
Mary {pad|4em} had a little lamb.
 
'''Badrul Arefin'''
 
 
# Jeong M^, {{spaces|7}} <u>Sun D^</u>, Luo M^, Huang Y, Challen GA, <u>Rodriguez B</u>, Zhang X, Chavez L, Wang H, Hannah R, Kim S, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, <u>Li W*</u>, Goodell MA*: Large conserved domains of low DNA methylation maintained by Dnmt3a. <br/> '''''          Nature Genetics''''' . Accepted.
# <u>Akdemir KC</u>, Jain AK, Alton K, Arronow B, J. CA, ..., <u>Li W*</u>, Barton MC*: Genome Wide Profiling Reveals Stimulus-specific Functions of p53 During Differentiation and DNA Damage of Human Embryonic Stem Cells. <br/>'''''Nucleic Acids Res.''''' Accepted
# Klein JB, Piao L, …, <u>Li W</u>, Shi X, Kutateladze TG: KDM5B demethylase binds to its histone target and the product through distinct modules.<br/>''Cell Reports''. Accepted. <br/><br/>'''2013''' <br/><br/>
# <u>Chen K</u>, <u>Xi Y</u>, Pan X, Dent S, He X, <u>Li W*</u>: DANPOS: Dynamic Analysis of Nucleosome Position and Occupancy by Sequencing. <br/>'''''Genome Res.''''' 23 (2013) 341-351. [http://www.bcm.edu/news/item.cfm?newsID=6723 BCM News];  [http://epigenie.com/danpos-reveals-dynamic-nucleosomes Epigenie Headline]  <br/><br/>
# <u>Wang L</u>, <u>Park JH</u>, Wang S, Kocher JP, <u>Li W*</u>: CPAT: Coding-Potential Assessment Tool Using an Alignment-Free Logistic Regression Model. <br/>'''''Nucleic Acids Res.''''' 41 (2013) e74.<br/><br/>
# <u>Lin X</u>, <u>Sun D</u>, <u>Rodriguez B</u>, Zhao Q, Sun H, Zhang Y, <u>Li W*</u>: BseQC: Quality Control of bisulfite sequencing experiments. <br/>'''''Bioinformatics''''' 29 (2013) 3227-3229. PMCID: PMC3842756 .<br/><br/>
# <u>Chen K^</u>, Wilson MA^, Hirsch C, Watson A, Liang S, Lu Y, <u>Li W*</u>, Dent S*: Stabilization of the Promoter Nucleosomes in Nucleosome Free Region by the Yeast Ssn6-Tup1 Corepressor. <br/>'''''Genome Res.''''' 23 (2013) 312-322. <br/><br/>

Revision as of 19:05, 9 August 2015


EmbryoName HK023- chicken embryo- Stage 19.5 - 8.12.14


Experiment

Objective To transform HB101s with a plasmid encoding GFP (called pGFP) and express this protein by inducing with arabinose. Testing out openwetware Procedure

  • Trigeminal nerve bilaterally DiO crystals
  • 7th/8th nerve bilaterally DiI crystals (Big chunk on 7th nerve on L side and big chunk on 8th on R side)

Notes No obvious contamination, purposelly put a greater chunk of dye on the 7th nerve on the L side and the 8th nerve on the R side so will see if there is any visible difference between the amount of neurons labelled in different positions within the rhombomere.

Files

Results

Facial

(Green)

  • Afferents


  • Efferents




VIII

(green)

  • Afferents


  • Efferents


  • Other


Basal plate

(red)


Summary

Questions

Future


<META HTTP-EQUIV="refresh" CONTENT="10;URL=http://my.newplace.com/">


Mary {pad|4em} had a little lamb.

Badrul Arefin


  1. Jeong M^, Template:Spaces Sun D^, Luo M^, Huang Y, Challen GA, Rodriguez B, Zhang X, Chavez L, Wang H, Hannah R, Kim S, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, Li W*, Goodell MA*: Large conserved domains of low DNA methylation maintained by Dnmt3a.
    Nature Genetics . Accepted.
  2. Akdemir KC, Jain AK, Alton K, Arronow B, J. CA, ..., Li W*, Barton MC*: Genome Wide Profiling Reveals Stimulus-specific Functions of p53 During Differentiation and DNA Damage of Human Embryonic Stem Cells.
    Nucleic Acids Res. Accepted
  3. Klein JB, Piao L, …, Li W, Shi X, Kutateladze TG: KDM5B demethylase binds to its histone target and the product through distinct modules.
    Cell Reports. Accepted.

    2013

  4. Chen K, Xi Y, Pan X, Dent S, He X, Li W*: DANPOS: Dynamic Analysis of Nucleosome Position and Occupancy by Sequencing.
    Genome Res. 23 (2013) 341-351. BCM News; Epigenie Headline

  5. Wang L, Park JH, Wang S, Kocher JP, Li W*: CPAT: Coding-Potential Assessment Tool Using an Alignment-Free Logistic Regression Model.
    Nucleic Acids Res. 41 (2013) e74.

  6. Lin X, Sun D, Rodriguez B, Zhao Q, Sun H, Zhang Y, Li W*: BseQC: Quality Control of bisulfite sequencing experiments.
    Bioinformatics 29 (2013) 3227-3229. PMCID: PMC3842756 .

  7. Chen K^, Wilson MA^, Hirsch C, Watson A, Liang S, Lu Y, Li W*, Dent S*: Stabilization of the Promoter Nucleosomes in Nucleosome Free Region by the Yeast Ssn6-Tup1 Corepressor.
    Genome Res. 23 (2013) 312-322.

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