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'''EmbryoName HK023- chicken embryo- Stage 19.5 - 8.12.14'''
<td class="tdboxes"><a href="http://openwetware.org/wiki/iGEM:Cambridge/2008/Notebook/Voltage"><img border="0" src="http://openwetware.org/images/0/00/Vol_logo.JPG" width="256" height="201"></a></td>
<td class="tdboxes"><a href="http://openwetware.org/wiki/iGEM:Cambridge/2008/Notebook/Voltage"><img border="0" src="http://openwetware.org/images/d/d1/Mag_logo.JPG" width="210" height="201"></a></td>
<td class="tdboxes"><a href="http://openwetware.org/wiki/iGEM:Cambridge/2008/Notebook/Voltage"><img border="0" src="http://openwetware.org/images/f/fe/Tur_logo.JPG" width="201" height="201"></a></td>
</html>


<hr width="810px">
<div id="about">
{{Cambridge08a}}
</div>
<hr width="810px">
__NOTOC__


==Experiment==


<html>
'''Objective'''
<table width="700" border="0" cellspacing="3">
To transform HB101s with a plasmid encoding GFP (called pGFP) and express this protein by inducing with arabinose. Testing out openwetware
   <tr>
'''Procedure'''
    <td width="200"><a href="http://www.labtech.co.uk/" class="noborder"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/labtech.jpg" alt="labtech" width="131" height="17"></a></td>
 
    <td width="200"><a href="http://www.clontech-europe.com/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/clontech.jpg" alt="Clontech" width="112" height="34"></a></td>
* Trigeminal nerve bilaterally DiO crystals
  <td width ="200"><a href="http://www.expressys.com/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/expressys.jpg" alt="expressys" width="112" height="70"></a></td>
* 7th/8th nerve bilaterally DiI crystals (Big chunk on 7th nerve on L side and big chunk on 8th on R side)
</tr>
 
  <tr>
'''Notes'''
  <td><a href="http://www.invitrogen.com/site/us/en/home.html"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/invirtogen.jpg" alt="invitrogen" width="160" height="25"></a></td>
No obvious contamination, purposelly put a greater chunk of dye on the 7th nerve on the L side and the 8th nerve on the R side so will see if there is any visible difference between the amount of neurons labelled in different positions within the rhombomere.
  <td><a href="http://www.medical-solutions.co.uk/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/geneservice.jpg" alt="geneservice" width="140" height="26"></a></td>
 
    <td><a href="http://www.bioscience.co.uk/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/cambridgebioscience.jpg" alt="cambridge bioscience" width="131" height="35"></a></td>
==Files==
</tr>
 
  <tr>
*
    <td><a href="http://www.zymoresearch.com/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/zymoresearch.jpg" alt="zymo research" width="135" height="45"></a></td>
*
    <td><a href="http://www.vwr.com/index.htm"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/vwr.jpg" alt="VWR" width="140" height="47"></a></td>
*
  <td><a href="http://www.microzone.co.uk/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/microzone.gif" alt="Microzone" width="131" height="44"></a></td>
*
</tr>
 
  <tr>
*
    <td><a href="http://www.finnzymes.com/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/finnzymes.jpg" alt="Finnzymes" width="131" height="43"></a></td>
**
      <td><a href="http://www.fisher.co.uk/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/fisherscientific.jpg" alt="Fisher Scientific" width="132" height="33" /></a></td>
**
    <td>&nbsp;</td>
 
  </tr>
==Results==
</table>
 
</div>
===Facial===
</html>
(Green)
 
* '''''Afferents'''''
    
 
* '''''Efferents'''''
 
 
 
 
 
 
===VIII ===
(green)
 
* '''''Afferents'''''
 
 
 
* '''''Efferents'''''
 
 
* '''''Other'''''
 
 
 
===Basal plate ===
(red)
 
 
==Summary==
 
'''Questions'''
 
'''Future'''
 
 
<META HTTP-EQUIV="refresh" CONTENT="10;URL=http://my.newplace.com/">
 
 
Mary {pad|4em} had a little lamb.
 
'''Badrul Arefin'''
 
 
# Jeong M^, {{spaces|7}} <u>Sun D^</u>, Luo M^, Huang Y, Challen GA, <u>Rodriguez B</u>, Zhang X, Chavez L, Wang H, Hannah R, Kim S, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, <u>Li W*</u>, Goodell MA*: Large conserved domains of low DNA methylation maintained by Dnmt3a. <br/> '''''          Nature Genetics''''' . Accepted.
# <u>Akdemir KC</u>, Jain AK, Alton K, Arronow B, J. CA, ..., <u>Li W*</u>, Barton MC*: Genome Wide Profiling Reveals Stimulus-specific Functions of p53 During Differentiation and DNA Damage of Human Embryonic Stem Cells. <br/>'''''Nucleic Acids Res.''''' Accepted
# Klein JB, Piao L, …, <u>Li W</u>, Shi X, Kutateladze TG: KDM5B demethylase binds to its histone target and the product through distinct modules.<br/>''Cell Reports''. Accepted. <br/><br/>'''2013''' <br/><br/>
# <u>Chen K</u>, <u>Xi Y</u>, Pan X, Dent S, He X, <u>Li W*</u>: DANPOS: Dynamic Analysis of Nucleosome Position and Occupancy by Sequencing. <br/>'''''Genome Res.''''' 23 (2013) 341-351. [http://www.bcm.edu/news/item.cfm?newsID=6723 BCM News];  [http://epigenie.com/danpos-reveals-dynamic-nucleosomes Epigenie Headline]  <br/><br/>
# <u>Wang L</u>, <u>Park JH</u>, Wang S, Kocher JP, <u>Li W*</u>: CPAT: Coding-Potential Assessment Tool Using an Alignment-Free Logistic Regression Model. <br/>'''''Nucleic Acids Res.''''' 41 (2013) e74.<br/><br/>
# <u>Lin X</u>, <u>Sun D</u>, <u>Rodriguez B</u>, Zhao Q, Sun H, Zhang Y, <u>Li W*</u>: BseQC: Quality Control of bisulfite sequencing experiments. <br/>'''''Bioinformatics''''' 29 (2013) 3227-3229. PMCID: PMC3842756 .<br/><br/>
# <u>Chen K^</u>, Wilson MA^, Hirsch C, Watson A, Liang S, Lu Y, <u>Li W*</u>, Dent S*: Stabilization of the Promoter Nucleosomes in Nucleosome Free Region by the Yeast Ssn6-Tup1 Corepressor. <br/>'''''Genome Res.''''' 23 (2013) 312-322. <br/><br/>

Revision as of 11:51, 25 March 2015

EmbryoName HK023- chicken embryo- Stage 19.5 - 8.12.14


Experiment

Objective To transform HB101s with a plasmid encoding GFP (called pGFP) and express this protein by inducing with arabinose. Testing out openwetware Procedure

  • Trigeminal nerve bilaterally DiO crystals
  • 7th/8th nerve bilaterally DiI crystals (Big chunk on 7th nerve on L side and big chunk on 8th on R side)

Notes No obvious contamination, purposelly put a greater chunk of dye on the 7th nerve on the L side and the 8th nerve on the R side so will see if there is any visible difference between the amount of neurons labelled in different positions within the rhombomere.

Files

Results

Facial

(Green)

  • Afferents


  • Efferents




VIII

(green)

  • Afferents


  • Efferents


  • Other


Basal plate

(red)


Summary

Questions

Future


<META HTTP-EQUIV="refresh" CONTENT="10;URL=http://my.newplace.com/">


Mary {pad|4em} had a little lamb.

Badrul Arefin


  1. Jeong M^, Template:Spaces Sun D^, Luo M^, Huang Y, Challen GA, Rodriguez B, Zhang X, Chavez L, Wang H, Hannah R, Kim S, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, Li W*, Goodell MA*: Large conserved domains of low DNA methylation maintained by Dnmt3a.
    Nature Genetics . Accepted.
  2. Akdemir KC, Jain AK, Alton K, Arronow B, J. CA, ..., Li W*, Barton MC*: Genome Wide Profiling Reveals Stimulus-specific Functions of p53 During Differentiation and DNA Damage of Human Embryonic Stem Cells.
    Nucleic Acids Res. Accepted
  3. Klein JB, Piao L, …, Li W, Shi X, Kutateladze TG: KDM5B demethylase binds to its histone target and the product through distinct modules.
    Cell Reports. Accepted.

    2013

  4. Chen K, Xi Y, Pan X, Dent S, He X, Li W*: DANPOS: Dynamic Analysis of Nucleosome Position and Occupancy by Sequencing.
    Genome Res. 23 (2013) 341-351. BCM News; Epigenie Headline

  5. Wang L, Park JH, Wang S, Kocher JP, Li W*: CPAT: Coding-Potential Assessment Tool Using an Alignment-Free Logistic Regression Model.
    Nucleic Acids Res. 41 (2013) e74.

  6. Lin X, Sun D, Rodriguez B, Zhao Q, Sun H, Zhang Y, Li W*: BseQC: Quality Control of bisulfite sequencing experiments.
    Bioinformatics 29 (2013) 3227-3229. PMCID: PMC3842756 .

  7. Chen K^, Wilson MA^, Hirsch C, Watson A, Liang S, Lu Y, Li W*, Dent S*: Stabilization of the Promoter Nucleosomes in Nucleosome Free Region by the Yeast Ssn6-Tup1 Corepressor.
    Genome Res. 23 (2013) 312-322.

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