Sandbox: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
(106 intermediate revisions by 42 users not shown)
Line 1: Line 1:
<div style="padding: 10px; color: #000000; background-color: #444444;">
__TOC__
<center>
[[Image:Xray_logo1.png|200px]]
</center>
</div><div style="padding: 10px; color: #000000; background-color: #444444;">
<center>
[[IGEM:Cambridge/2008| <font face="verdana" style="color:#ffffff"> '''Home''' </font>]] &nbsp;
[[iGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation| <font face="verdana" style="color:#b3c6fe"> '''Signalling''' </font>]]&nbsp;
[[IGEM:Cambridge/2008/Notebook/Bacillus| <font face="verdana" style="color:#b3c6fe"> '''Bacillus''' </font>]]&nbsp;
[[iGEM:Cambridge/2008/Notebook/Voltage| <font face="verdana" style="color:#b3c6fe"> '''Voltage'''
</font>]]&nbsp;
[[iGEM:Cambridge/2008/Notebook/Modelling| <font face="verdana" style="color:#b3c6fe"> '''Modelling''' </font>]]&nbsp;
[[IGEM:Cambridge/2008/Protocols| <font face="verdana" style="color:#ffffff"> '''Protocols''' </font>]]&nbsp;
[[IGEM:Cambridge/2008/Members| <font face="verdana" style="color:#ffffff"> '''People''' </font>]]&nbsp;
</center>
</div>


<html xmlns:o="urn:schemas-microsoft-com:office:office"
'''EmbryoName HK023- chicken embryo- Stage 19.5 - 8.12.14'''
xmlns:x="urn:schemas-microsoft-com:office:excel"
xmlns="http://www.w3.org/TR/REC-html40">


<head>
<meta http-equiv=Content-Type content="text/html; charset=windows-1252">
<meta name=ProgId content=Excel.Sheet>
<meta name=Generator content="Microsoft Excel 11">
<link rel=File-List href="Parts_files/filelist.xml">
<style id="Extracted parts_32375_Styles"><!--table
{mso-displayed-decimal-separator:"\.";
mso-displayed-thousand-separator:"\,";}
.xl1532375
{padding-top:1px;
padding-right:1px;
padding-left:1px;
mso-ignore:padding;
color:windowtext;
font-size:10.0pt;
font-weight:400;
font-style:normal;
text-decoration:none;
font-family:Arial;
mso-generic-font-family:auto;
mso-font-charset:0;
mso-number-format:General;
text-align:general;
vertical-align:bottom;
mso-background-source:auto;
mso-pattern:auto;
white-space:nowrap;}
.xl2432375
{padding-top:1px;
padding-right:1px;
padding-left:1px;
mso-ignore:padding;
color:windowtext;
font-size:10.0pt;
font-weight:700;
font-style:normal;
text-decoration:none;
font-family:Arial, sans-serif;
mso-font-charset:0;
mso-number-format:General;
text-align:general;
vertical-align:bottom;
border:.5pt solid windowtext;
mso-background-source:auto;
mso-pattern:auto;
white-space:normal;}
.xl2532375
{padding-top:1px;
padding-right:1px;
padding-left:1px;
mso-ignore:padding;
color:windowtext;
font-size:10.0pt;
font-weight:700;
font-style:normal;
text-decoration:none;
font-family:Arial, sans-serif;
mso-font-charset:0;
mso-number-format:General;
text-align:general;
vertical-align:bottom;
border:.5pt solid windowtext;
mso-background-source:auto;
mso-pattern:auto;
white-space:nowrap;}
.xl2632375
{padding-top:1px;
padding-right:1px;
padding-left:1px;
mso-ignore:padding;
color:windowtext;
font-size:10.0pt;
font-weight:400;
font-style:normal;
text-decoration:none;
font-family:Arial;
mso-generic-font-family:auto;
mso-font-charset:0;
mso-number-format:General;
text-align:general;
vertical-align:bottom;
border:.5pt solid windowtext;
mso-background-source:auto;
mso-pattern:auto;
white-space:nowrap;}
.xl2732375
{padding-top:1px;
padding-right:1px;
padding-left:1px;
mso-ignore:padding;
color:windowtext;
font-size:10.0pt;
font-weight:400;
font-style:normal;
text-decoration:none;
font-family:Arial, sans-serif;
mso-font-charset:0;
mso-number-format:General;
text-align:general;
vertical-align:bottom;
border:.5pt solid windowtext;
mso-background-source:auto;
mso-pattern:auto;
white-space:nowrap;}
.xl2832375
{padding-top:1px;
padding-right:1px;
padding-left:1px;
mso-ignore:padding;
color:blue;
font-size:10.0pt;
font-weight:400;
font-style:normal;
text-decoration:underline;
text-underline-style:single;
font-family:Arial;
mso-generic-font-family:auto;
mso-font-charset:0;
mso-number-format:General;
text-align:general;
vertical-align:bottom;
border:.5pt solid windowtext;
mso-background-source:auto;
mso-pattern:auto;
white-space:nowrap;}
--></style>
<title>Extracted BioBrick Parts</title>
</head>


<body>
==Experiment==
<!--[if !excel]>&nbsp;&nbsp;<![endif]-->
<!--The following information was generated by Microsoft Office Excel's Publish
as Web Page wizard.-->
<!--If the same item is republished from Excel, all information between the DIV
tags will be replaced.-->


<!----------------------------->
'''Objective'''
<!--START OF OUTPUT FROM EXCEL PUBLISH AS WEB PAGE WIZARD -->
To transform HB101s with a plasmid encoding GFP (called pGFP) and express this protein by inducing with arabinose.
<!----------------------------->
'''Procedure'''


<div id="Extracted parts_32375" align=center x:publishsource="Excel">
* Trigeminal nerve bilaterally DiO crystals
* 7th/8th nerve bilaterally DiI crystals (Big chunk on 7th nerve on L side and big chunk on 8th on R side)


<h1 style='color:black;font-family:Arial;font-size:14.0pt;font-weight:800;
'''Notes'''
font-style:normal'>Extracted BioBrick Parts</h1>
No obvious contamination, purposelly put a greater chunk of dye on the 7th nerve on the L side and the 8th nerve on the R side so will see if there is any visible difference between the amount of neurons labelled in different positions within the rhombomere.  


<table x:str border=0 cellpadding=0 cellspacing=0 width=708 style='border-collapse:
==Files==
collapse;table-layout:fixed;width:531pt'>
<col width=83 style='mso-width-source:userset;mso-width-alt:3035;width:62pt'>
<col width=437 style='mso-width-source:userset;mso-width-alt:15981;width:328pt'>
<col width=116 style='mso-width-source:userset;mso-width-alt:4242;width:87pt'>
<col width=72 style='mso-width-source:userset;mso-width-alt:2633;width:54pt'>


<tr height=17 style='height:12.75pt'>
*
  <td height=17 class=xl2432375 width=83 style='height:12.75pt;width:62pt'>Part
*
  No</td>
*
  <td class=xl2532375 width=437 style='border-left:none;width:328pt'>Description</td>
*
  <td class=xl2532375 width=116 style='border-left:none;width:87pt'>Plasmid</td>
  <td class=xl2532375 width=72 style='border-left:none;width:54pt'>Resistance</td>
</tr>
<tr height=17 style='height:12.75pt'>


  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>B1002</td>
*
  <td class=xl2632375 style='border-top:none;border-left:none'>Stop</td>
**
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1AK3</td>
**
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>B1006</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>Stop</td>
==Results==
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1AK3</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>J45995</td>
  <td class=xl2732375 style='border-top:none;border-left:none'>Stationary phase
  dependent GFP generator</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1AT3</td>
===Facial===
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
(Green)
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>B0030</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>RBS strong</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
* '''''Afferents'''''
</tr>
    
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>E0840</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>GFP</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2</td>
   <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>


</tr>
* '''''Efferents'''''
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>J23114</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>Promoter</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>J61002</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>


<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>R0040</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>TetR repressible
  promoter</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>


  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>J23113</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>Promoter</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>J61002</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>E0040</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>GFP</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>I732007</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>BgIII and BamHI</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A3</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>J37016</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>Lux R GFP</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>J23100</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>Promoter</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>J61002</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>


</tr>
===VIII ===
<tr height=17 style='height:12.75pt'>
(green)
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>J45996</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>Anti-OsmY GFP</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1AK3</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>


<tr height=17 style='height:12.75pt'>
* '''''Afferents'''''
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>H21001</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>I13522</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>pTET GFP</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2 or J23006</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>I13522('07)</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pTET GFP</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2 or J23006</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>C0012</td>
  <td class=xl2832375 style='border-top:none;border-left:none'>LacI repressor</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
* '''''Efferents'''''
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>I714062</td>
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:BBa%20I714062">GFP Stop</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>


</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>Q04121</td>
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:BBa%20Q04121">QPI</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB2K3</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>


<tr height=17 style='height:12.75pt'>
* '''''Other'''''
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>F1610</td>
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:BBa%20F1610">OHL sender</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1AK3</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>


  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>J04630</td>
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:BBa%20J04630">GFP stop</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1AK3</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>C0061</td>


  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:BBa%20C0061">AHL synthase</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1A2</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>B0014</td>
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:BBa%20B0014">Stop Stop</a></td>


  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1AK3</td>
===Basal plate ===
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
(red)
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>I40630('07)</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>


</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>B0015</td>
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:BBa%20B0015">Stop</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>pSB1AK3</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>


<tr height=17 style='height:12.75pt'>
==Summary==
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>I45250</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>&nbsp;</td>


  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
'''Questions'''
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>


</tr>
'''Future'''
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>pSB1AK3</td>


  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:pSB1AK3">High copy number plasmid
  carrying ampicillin and kanamycin resistance</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>pSB1A2</td>
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:pSB1A2">High copy number plasmid carrying
  ampicillin resistance.</a></td>


  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
<META HTTP-EQUIV="refresh" CONTENT="10;URL=http://my.newplace.com/">
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>pSB1A3</td>
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:pSB1A3">high copy number plasmid carrying
  ampicillin resistance.</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>


</tr>
<tr height=17 style='height:12.75pt'>
  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>pSB2K3</td>
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:pSB2K3">variable copy number plasmid
  carrying kanamycin resistance</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<tr height=17 style='height:12.75pt'>


  <td height=17 class=xl2632375 style='height:12.75pt;border-top:none'>J23006</td>
Mary {pad|4em} had a little lamb.
  <td class=xl2832375 style='border-top:none;border-left:none'><a
  href="http://partsregistry.org/Part:BBa%20J23006">Plaasmid which puts
  biobrick uder pTET</a></td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
  <td class=xl2632375 style='border-top:none;border-left:none'>&nbsp;</td>
</tr>
<![if supportMisalignedColumns]>
<tr height=0 style='display:none'>
  <td width=83 style='width:62pt'></td>


  <td width=437 style='width:328pt'></td>
'''Badrul Arefin'''
  <td width=116 style='width:87pt'></td>
  <td width=72 style='width:54pt'></td>
</tr>
<![endif]>
</table>


</div>


 
# Jeong M^, {{spaces|7}} <u>Sun D^</u>, Luo M^, Huang Y, Challen GA, <u>Rodriguez B</u>, Zhang X, Chavez L, Wang H, Hannah R, Kim S, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, <u>Li W*</u>, Goodell MA*: Large conserved domains of low DNA methylation maintained by Dnmt3a. <br/> '''''          Nature Genetics''''' . Accepted.
<!----------------------------->
# <u>Akdemir KC</u>, Jain AK, Alton K, Arronow B, J. CA, ..., <u>Li W*</u>, Barton MC*: Genome Wide Profiling Reveals Stimulus-specific Functions of p53 During Differentiation and DNA Damage of Human Embryonic Stem Cells. <br/>'''''Nucleic Acids Res.''''' Accepted
<!--END OF OUTPUT FROM EXCEL PUBLISH AS WEB PAGE WIZARD-->
# Klein JB, Piao L, …, <u>Li W</u>, Shi X, Kutateladze TG: KDM5B demethylase binds to its histone target and the product through distinct modules.<br/>''Cell Reports''. Accepted. <br/><br/>'''2013''' <br/><br/>
 
# <u>Chen K</u>, <u>Xi Y</u>, Pan X, Dent S, He X, <u>Li W*</u>: DANPOS: Dynamic Analysis of Nucleosome Position and Occupancy by Sequencing. <br/>'''''Genome Res.''''' 23 (2013) 341-351. [http://www.bcm.edu/news/item.cfm?newsID=6723 BCM News];  [http://epigenie.com/danpos-reveals-dynamic-nucleosomes Epigenie Headline]  <br/><br/>
<!----------------------------->
# <u>Wang L</u>, <u>Park JH</u>, Wang S, Kocher JP, <u>Li W*</u>: CPAT: Coding-Potential Assessment Tool Using an Alignment-Free Logistic Regression Model. <br/>'''''Nucleic Acids Res.''''' 41 (2013) e74.<br/><br/>
</body>
# <u>Lin X</u>, <u>Sun D</u>, <u>Rodriguez B</u>, Zhao Q, Sun H, Zhang Y, <u>Li W*</u>: BseQC: Quality Control of bisulfite sequencing experiments. <br/>'''''Bioinformatics''''' 29 (2013) 3227-3229. PMCID: PMC3842756 .<br/><br/>
 
# <u>Chen K^</u>, Wilson MA^, Hirsch C, Watson A, Liang S, Lu Y, <u>Li W*</u>, Dent S*: Stabilization of the Promoter Nucleosomes in Nucleosome Free Region by the Yeast Ssn6-Tup1 Corepressor. <br/>'''''Genome Res.''''' 23 (2013) 312-322. <br/><br/>
</html>

Revision as of 13:00, 21 December 2014

EmbryoName HK023- chicken embryo- Stage 19.5 - 8.12.14


Experiment

Objective To transform HB101s with a plasmid encoding GFP (called pGFP) and express this protein by inducing with arabinose. Procedure

  • Trigeminal nerve bilaterally DiO crystals
  • 7th/8th nerve bilaterally DiI crystals (Big chunk on 7th nerve on L side and big chunk on 8th on R side)

Notes No obvious contamination, purposelly put a greater chunk of dye on the 7th nerve on the L side and the 8th nerve on the R side so will see if there is any visible difference between the amount of neurons labelled in different positions within the rhombomere.

Files

Results

Facial

(Green)

  • Afferents


  • Efferents




VIII

(green)

  • Afferents


  • Efferents


  • Other


Basal plate

(red)


Summary

Questions

Future


<META HTTP-EQUIV="refresh" CONTENT="10;URL=http://my.newplace.com/">


Mary {pad|4em} had a little lamb.

Badrul Arefin


  1. Jeong M^, Template:Spaces Sun D^, Luo M^, Huang Y, Challen GA, Rodriguez B, Zhang X, Chavez L, Wang H, Hannah R, Kim S, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, Li W*, Goodell MA*: Large conserved domains of low DNA methylation maintained by Dnmt3a.
    Nature Genetics . Accepted.
  2. Akdemir KC, Jain AK, Alton K, Arronow B, J. CA, ..., Li W*, Barton MC*: Genome Wide Profiling Reveals Stimulus-specific Functions of p53 During Differentiation and DNA Damage of Human Embryonic Stem Cells.
    Nucleic Acids Res. Accepted
  3. Klein JB, Piao L, …, Li W, Shi X, Kutateladze TG: KDM5B demethylase binds to its histone target and the product through distinct modules.
    Cell Reports. Accepted.

    2013

  4. Chen K, Xi Y, Pan X, Dent S, He X, Li W*: DANPOS: Dynamic Analysis of Nucleosome Position and Occupancy by Sequencing.
    Genome Res. 23 (2013) 341-351. BCM News; Epigenie Headline

  5. Wang L, Park JH, Wang S, Kocher JP, Li W*: CPAT: Coding-Potential Assessment Tool Using an Alignment-Free Logistic Regression Model.
    Nucleic Acids Res. 41 (2013) e74.

  6. Lin X, Sun D, Rodriguez B, Zhao Q, Sun H, Zhang Y, Li W*: BseQC: Quality Control of bisulfite sequencing experiments.
    Bioinformatics 29 (2013) 3227-3229. PMCID: PMC3842756 .

  7. Chen K^, Wilson MA^, Hirsch C, Watson A, Liang S, Lu Y, Li W*, Dent S*: Stabilization of the Promoter Nucleosomes in Nucleosome Free Region by the Yeast Ssn6-Tup1 Corepressor.
    Genome Res. 23 (2013) 312-322.