Sack and Yarov-Yarovoy:Protocols: Difference between revisions
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[[Sack: Freezing CHO cells|Freezing CHO Cells 04/05/12]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf .]<BR> | [[Sack: Freezing CHO cells|Freezing CHO Cells 04/05/12]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf .]<BR> | ||
[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys 09/29/11]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf .]<BR> | [[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys 09/29/11]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf .]<BR> | ||
[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for | [[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Fluorescent Screening 04/05/12]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf .]<BR> | ||
[[Sack: Subcloning Cells| Subcloning Cells 02/23/11]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf .]<BR> | [[Sack: Subcloning Cells| Subcloning Cells 02/23/11]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf .]<BR> | ||
[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys 04/06/11]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf .]<BR> | [[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys 04/06/11]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf .]<BR> | ||
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==Ephys== | ==Ephys== | ||
[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf .]<br> | [[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf .]<br> | ||
== | ==Fluorophore Labeling== | ||
[[Sack: GxTx Purification|GxTx Purification 11/22/11]][http://openwetware.org/images/1/1f/112211_OMIXC18_GxTX_purification.pdf .]<br> | [[Sack: GxTx Purification|GxTx Purification 11/22/11]][http://openwetware.org/images/1/1f/112211_OMIXC18_GxTX_purification.pdf .]<br> | ||
[[Sack: Alyknefluor Azide Beads|Alyknefluor Azide Beads 11/11/11]][http://openwetware.org/images/9/9b/Alyknefluor_Azide_beads_111111.pdf .]<br> | [[Sack: Alyknefluor Azide Beads|Alyknefluor Azide Beads 11/11/11]][http://openwetware.org/images/9/9b/Alyknefluor_Azide_beads_111111.pdf .]<br> | ||
Revision as of 11:54, 25 October 2012
Cell Culture
Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12 .
Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11 .
Cell Splitting Protocol 03/02/12.
Cell Transfection (Lipofectamine LTX) 05/01/12.
Cell Transfection (Lipofectamine) 08/14/12.
Cell Transfection (Novachoice) 05/04/12.
Cell Harvesting for Electrophysiology 07/18/12.
Cell Plating for Electrophysiology 10/21/11 .
Freezing CHO Cells 04/05/12.
Plating Subclones for Ephys 09/29/11.
Plating Subclones for Fluorescent Screening 04/05/12.
Subcloning Cells 02/23/11.
Testing TREX Subclones Ephys 04/06/11.
Thawing CHO cells 07/09/12.
Ephys
Fluorophore Labeling
GxTx Purification 11/22/11.
Alyknefluor Azide Beads 11/11/11.
Maleimide Labeling 11/08/11.
Maleimide Labeling TAMRA 10/08/12.
General Lab
HPLC
BTAA Peptide Click HPLC 01/03/12.
Dylight Protocol 02/06/12.
Fmal Protocol.
HPLC Protocol.
Mass Spec Protocol for GxTx-13ACM.
Solutions
Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.
Molecular Biology
Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .
Tentagel Azide Beads
Bead Cell Binding 05/15/12.
Beads Alykne Azide BTAA 05/16/12.
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