Sack and Yarov-Yarovoy:Protocols

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==Cell Culture==
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{{Template: Sack Lab}}
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf  .]<br>
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf  .]<br>
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[[Sack: Cell Splitting Protocol|Cell Splitting Protocol 03/02/12]][http://openwetware.org/images/a/ac/Cell_Splitting_Protocol.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX) 05/01/12]][http://openwetware.org/images/e/e4/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine) 08/14/12]][http://openwetware.org/images/3/37/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.pdf .]<BR>
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[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice) 05/04/12]][http://openwetware.org/images/6/61/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.pdf  .]<BR>
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[[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology 07/18/12]][http://openwetware.org/images/4/48/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.pdf  .]<br>
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[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology 10/21/11]] [http://openwetware.org/images/1/1c/Cell_Plating_for_Electrophysiology_SackLab.pdf  .]<br>
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[[Sack: Freezing CHO cells|Freezing CHO Cells 04/05/12]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys 09/29/11]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Fluorescent Screening 04/05/12]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf  .]<BR>
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[[Sack: Subcloning Cells| Subcloning Cells 02/23/11]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf  .]<BR>
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[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys 04/06/11]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf  .]<BR>
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[[Sack: Thawing CHOcells| Thawing CHO cells 07/09/12]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf  .]<BR>
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==Ephys==
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<html>
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[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf  .]<br>
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<head>
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<meta http-equiv="Refresh" content="0; url=https://basicscience.ucdmc.ucdavis.edu/Sack_and_Yarov-Yarovoy_Labs/" />
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</head>
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</html>
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<div style="right: center; text-align: center; float:none">
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[[Image:C Guanxiensis 1.tif|400px]]
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<div style="right: center; text-align: left; float:none">
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==General Lab==
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==general lab==
[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf  .]<br>
[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf  .]<br>
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==HPLC==
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==cell culture==
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[[Sack: BTAA Peptide Click HPLC|BTAA Peptide Click HPLC 01/03/12]][http://openwetware.org/images/f/ff/BTTAA_peptide_click_HPLC_010312.pdf   .]<br>
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf  .]<br>
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[[Sack: Dylight Protocol|Dylight Protocol 02/06/12]][http://openwetware.org/images/3/32/Dylight_protocol_02_06_2012_jon.pdf  .]<br>
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin)]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf .]<br>
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[[Sack: Fmal Protocol|Fmal Protocol]][http://openwetware.org/images/5/50/Fmal_protocol_03_07_2012.pdf  .]<br>
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[[Sack: Cell Splitting Protocol|Cell Splitting Protocol]][http://openwetware.org/images/a/ac/Cell_Splitting_Protocol.pdf  .]<br>
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[[Sack: HPLC Protocol|HPLC Protocol]][http://openwetware.org/images/d/d2/HPLC.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX)]][http://openwetware.org/images/e/e4/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.pdf  .]<br>
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[[Sack: Mass Spec Protocol for GxTx-13ACM|Mass Spec Protocol for GxTx-13ACM]][http://openwetware.org/images/f/f4/Mass_Spec_Protocol_for_GxTx.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine)]][http://openwetware.org/images/3/37/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.pdf .]<BR>
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[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice)]][http://openwetware.org/images/6/61/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.pdf  .]<BR>
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[[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology]][http://openwetware.org/images/4/48/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.pdf  .]<br>
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[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology]] [http://openwetware.org/images/1/1c/Cell_Plating_for_Electrophysiology_SackLab.pdf  .]<br>
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[[Sack: Freezing CHO cells|Freezing CHO Cells]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Fluorescent Screening]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf  .]<BR>
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[[Sack: Subcloning Cells| Subcloning Cells]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf  .]<BR>
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[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf  .]<BR>
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[[Sack: Thawing CHOcells| Thawing CHO cells]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf  .]<BR>
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==electrophys==
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[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf  .]<br>
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==Solutions==
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==molecular biology==
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Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx  .]<br>
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Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx  .]<br>
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Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls  .]<br>
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Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>
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==Molecular Biology==
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[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf  .]<br>
[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf  .]<br>
Bacteria Transformation Invitrogen  [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf  .]<br>
Bacteria Transformation Invitrogen  [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf  .]<br>
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Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
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==solutions==
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Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx  .]<br>
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Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx  .]<br>
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Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls  .]<br>
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Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>
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==Tentagel Azide Beads==
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==ELISAs==
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[[Sack: Bead Cell Binding| Bead Cell Binding 05/15/12]][http://openwetware.org/images/f/f0/10mm_dish_cell_scraping_protocol.pdf  .]<br>
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[[ELISA Protocol for a 96-well Plate]][http://openwetware.org/images/5/54/Elisa_protocol_for_online.pdf  .]<br>
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[[Sack: Beads Alykne Azide BTAA| Beads Alykne Azide BTTAA 05/16/12]][http://openwetware.org/images/1/17/Beads_alykne_Azide_BTTAA_gxtx_051612-1.pdf  .]<br>
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ELISA Spreadsheet Skeleton  [http://openwetware.org/images/7/7a/2012-11-06_ELISA_layout.xlsx    .]
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*Return to [[Sack]] Homepage
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Current revision


Sack and Yarov-Yarovoy Labs

Department of Physiology and Membrane Biology
University of California, Davis

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general lab

Acidwash 03/22/11.

cell culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) .
Cell Media with Selection Agents (Blasticidin, Zeocin) .
Cell Splitting Protocol.
Cell Transfection (Lipofectamine LTX).
Cell Transfection (Lipofectamine).
Cell Transfection (Novachoice).
Cell Harvesting for Electrophysiology.
Cell Plating for Electrophysiology .
Freezing CHO Cells.
Plating Subclones for Ephys.
Plating Subclones for Fluorescent Screening.
Subcloning Cells.
Testing TREX Subclones Ephys.
Thawing CHO cells.

electrophys

Agar Bridge Formation.

molecular biology

Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .

solutions

Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.

ELISAs

ELISA Protocol for a 96-well Plate.

ELISA Spreadsheet Skeleton .
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