Sack and Yarov-Yarovoy:Protocols

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==Cell Culture==
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{{Template: Sack Lab}}
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf  .]<br>
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf  .]<br>
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[[Sack: Cell Splitting Protocol|Cell Splitting Protocol 03/02/12]][http://openwetware.org/images/a/ac/Cell_Splitting_Protocol.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX) 05/01/12]][http://openwetware.org/images/e/e4/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine) 08/14/12]][http://openwetware.org/images/3/37/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.pdf .]<BR>
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[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice) 05/04/12]][http://openwetware.org/images/6/61/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.pdf  .]<BR>
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[[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology 07/18/12]][http://openwetware.org/images/4/48/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.pdf  .]<br>
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[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology 10/21/11]] [http://openwetware.org/images/1/1c/Cell_Plating_for_Electrophysiology_SackLab.pdf  .]<br>
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[[Sack: Freezing CHO cells|Freezing CHO Cells 04/05/12]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys 09/29/11]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Flourescent Screening 04/05/12]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf  .]<BR>
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[[Sack: Subcloning Cells| Subcloning Cells 02/23/11]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf  .]<BR>
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[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys 04/06/11]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf  .]<BR>
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[[Sack: Thawing CHOcells| Thawing CHO cells 07/09/12]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf  .]<BR>
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==Ephys==
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<html>
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[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf  .]<br>
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<head>
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<meta http-equiv="Refresh" content="0; url=https://basicscience.ucdmc.ucdavis.edu/Sack_and_Yarov-Yarovoy_Labs/" />
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</head>
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</html>
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==Flourophore Labeling==
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<div style="right: center; text-align: center; float:none">
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[[Sack: GxTx Purification|GxTx Purification 11/22/11]][http://openwetware.org/images/1/1f/112211_OMIXC18_GxTX_purification.pdf  .]<br>  
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[[Image:C Guanxiensis 1.tif|400px]]
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[[Sack: Alyknefluor Azide Beads|Alyknefluor Azide Beads 11/11/11]][http://openwetware.org/images/9/9b/Alyknefluor_Azide_beads_111111.pdf  .]<br>
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<div style="right: center; text-align: left; float:none">
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[[Sack: Maleimide Labeling| Maleimide Labeling 11/08/11]][http://openwetware.org/images/2/23/Maleimide_labeling_110811jon.pdf  .]<br>
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[[Sack: Maleimide Labeling TAMRA| Maleimide Labeling TAMRA 10/08/12]][http://openwetware.org/images/0/03/Maleimide_labeling_TAMRA_121411SA.pdf  .]<br>
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==general lab==
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==General Lab==
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[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf  .]<br>
[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf  .]<br>
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==cell culture==
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf  .]<br>
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[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin)]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf  .]<br>
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[[Sack: Cell Splitting Protocol|Cell Splitting Protocol]][http://openwetware.org/images/a/ac/Cell_Splitting_Protocol.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX)]][http://openwetware.org/images/e/e4/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.pdf  .]<br>
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[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine)]][http://openwetware.org/images/3/37/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.pdf .]<BR>
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[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice)]][http://openwetware.org/images/6/61/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.pdf  .]<BR>
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[[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology]][http://openwetware.org/images/4/48/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.pdf  .]<br>
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[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology]] [http://openwetware.org/images/1/1c/Cell_Plating_for_Electrophysiology_SackLab.pdf  .]<br>
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[[Sack: Freezing CHO cells|Freezing CHO Cells]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf  .]<BR>
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[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Fluorescent Screening]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf  .]<BR>
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[[Sack: Subcloning Cells| Subcloning Cells]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf  .]<BR>
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[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf  .]<BR>
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[[Sack: Thawing CHOcells| Thawing CHO cells]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf  .]<BR>
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==electrophys==
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[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf  .]<br>
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==molecular biology==
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[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf  .]<br>
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Bacteria Transformation Invitrogen  [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf  .]<br>
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Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
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Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
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==solutions==
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Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx  .]<br>
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Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx  .]<br>
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Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls  .]<br>
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Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>
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*Return to [[Sack]] Homepage
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==ELISAs==
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[[ELISA Protocol for a 96-well Plate]][http://openwetware.org/images/5/54/Elisa_protocol_for_online.pdf  .]<br>
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ELISA Spreadsheet Skeleton  [http://openwetware.org/images/7/7a/2012-11-06_ELISA_layout.xlsx    .]

Current revision


Sack and Yarov-Yarovoy Labs

Department of Physiology and Membrane Biology
University of California, Davis

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general lab

Acidwash 03/22/11.

cell culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) .
Cell Media with Selection Agents (Blasticidin, Zeocin) .
Cell Splitting Protocol.
Cell Transfection (Lipofectamine LTX).
Cell Transfection (Lipofectamine).
Cell Transfection (Novachoice).
Cell Harvesting for Electrophysiology.
Cell Plating for Electrophysiology .
Freezing CHO Cells.
Plating Subclones for Ephys.
Plating Subclones for Fluorescent Screening.
Subcloning Cells.
Testing TREX Subclones Ephys.
Thawing CHO cells.

electrophys

Agar Bridge Formation.

molecular biology

Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .

solutions

Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.

ELISAs

ELISA Protocol for a 96-well Plate.

ELISA Spreadsheet Skeleton .
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