Sack and Yarov-Yarovoy:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (19:27, 15 January 2013) (view source)
(→ELISAs)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
<br>
+
{{Template: Sack Lab}}<br>
<div style="right: center; text-align: center; float:none">
<div style="right: center; text-align: center; float:none">
[[Image:C Guanxiensis 1.tif|400px]]
[[Image:C Guanxiensis 1.tif|400px]]
-
 
-
 
<div style="right: center; text-align: left; float:none">
<div style="right: center; text-align: left; float:none">
Line 39: Line 37:
Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls  .]<br>
Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls  .]<br>
Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>
Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>
 +
 +
==ELISAs==
 +
[[ELISA Protocol for a 96-well Plate]][http://openwetware.org/images/5/54/Elisa_protocol_for_online.pdf  .]<br>
 +
ELISA Spreadsheet Skeleton  [http://openwetware.org/images/5/5b/Elisa_skeleton.xlsx    .]

Current revision


Sack and Yarov-Yarovoy Labs

Department of Physiology and Membrane Biology
University of California, Davis

Home        Research        People        Contact        Protocols        Resources       



general lab

Acidwash 03/22/11.

cell culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) .
Cell Media with Selection Agents (Blasticidin, Zeocin) .
Cell Splitting Protocol.
Cell Transfection (Lipofectamine LTX).
Cell Transfection (Lipofectamine).
Cell Transfection (Novachoice).
Cell Harvesting for Electrophysiology.
Cell Plating for Electrophysiology .
Freezing CHO Cells.
Plating Subclones for Ephys.
Plating Subclones for Fluorescent Screening.
Subcloning Cells.
Testing TREX Subclones Ephys.
Thawing CHO cells.

electrophys

Agar Bridge Formation.

molecular biology

Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .

solutions

Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.

ELISAs

ELISA Protocol for a 96-well Plate.

ELISA Spreadsheet Skeleton .
Retrieved from "http://openwetware.org/wiki/Sack_and_Yarov-Yarovoy:Protocols"
Personal tools