Sack and Yarov-Yarovoy:Protocols

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{{Template: Sack Lab}}<br>
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<div style="right: center; text-align: center; float:none">
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[[Image:C Guanxiensis 1.tif|400px]]
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<div style="right: center; text-align: left; float:none">
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==general lab==
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[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf  .]<br>
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==cell culture==
==cell culture==
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf  .]<br>
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf  .]<br>
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[[Sack: Thawing CHOcells| Thawing CHO cells]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf  .]<BR>
[[Sack: Thawing CHOcells| Thawing CHO cells]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf  .]<BR>
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==electrophysiology==
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==electrophys==
[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf  .]<br>
[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf  .]<br>
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==general lab==
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==molecular biology==
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[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf   .]<br>
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[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf .]<br>
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Bacteria Transformation Invitrogen  [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf .]<br>
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==HPLC==
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Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
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[[Sack: BTAA Peptide Click HPLC|BTAA Peptide Click HPLC 01/03/12]][http://openwetware.org/images/f/ff/BTTAA_peptide_click_HPLC_010312.pdf   .]<br>
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Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
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[[Sack: Dylight Protocol|Dylight Protocol 02/06/12]][http://openwetware.org/images/3/32/Dylight_protocol_02_06_2012_jon.pdf  .]<br>
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[[Sack: Fmal Protocol|Fmal Protocol]][http://openwetware.org/images/5/50/Fmal_protocol_03_07_2012.pdf  .]<br>
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[[Sack: HPLC Protocol|HPLC Protocol]][http://openwetware.org/images/d/d2/HPLC.pdf  .]<br>
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[[Sack: Mass Spec Protocol for GxTx-13ACM|Mass Spec Protocol for GxTx-13ACM]][http://openwetware.org/images/f/f4/Mass_Spec_Protocol_for_GxTx.pdf  .]<br>
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==Solutions==
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==solutions==
Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx  .]<br>
Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx  .]<br>
Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx  .]<br>
Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx  .]<br>
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Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>
Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>
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==ELISAs==
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==molecular biology==
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[[ELISA Protocol for a 96-well Plate]][http://openwetware.org/images/5/54/Elisa_protocol_for_online.pdf   .]<br>
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[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf .]<br>
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ELISA Spreadsheet Skeleton [http://openwetware.org/images/5/5b/Elisa_skeleton.xlsx    .]
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Bacteria Transformation Invitrogen [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf  .]<br>
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Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
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Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
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Revision as of 19:27, 15 January 2013


Sack and Yarov-Yarovoy Labs

Department of Physiology and Membrane Biology
University of California, Davis

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general lab

Acidwash 03/22/11.

cell culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) .
Cell Media with Selection Agents (Blasticidin, Zeocin) .
Cell Splitting Protocol.
Cell Transfection (Lipofectamine LTX).
Cell Transfection (Lipofectamine).
Cell Transfection (Novachoice).
Cell Harvesting for Electrophysiology.
Cell Plating for Electrophysiology .
Freezing CHO Cells.
Plating Subclones for Ephys.
Plating Subclones for Fluorescent Screening.
Subcloning Cells.
Testing TREX Subclones Ephys.
Thawing CHO cells.

electrophys

Agar Bridge Formation.

molecular biology

Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .

solutions

Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.

ELISAs

ELISA Protocol for a 96-well Plate.

ELISA Spreadsheet Skeleton .
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