Sack and Yarov-Yarovoy:Protocols
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| - | == | + | ==general lab== |
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[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf .]<br> | [[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf .]<br> | ||
| + | ==cell culture== | ||
| + | [[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf .]<br> | ||
| + | [[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin)]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf .]<br> | ||
| + | [[Sack: Cell Splitting Protocol|Cell Splitting Protocol]][http://openwetware.org/images/a/ac/Cell_Splitting_Protocol.pdf .]<br> | ||
| + | [[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX)]][http://openwetware.org/images/e/e4/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.pdf .]<br> | ||
| + | [[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine)]][http://openwetware.org/images/3/37/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.pdf .]<BR> | ||
| + | [[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice)]][http://openwetware.org/images/6/61/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.pdf .]<BR> | ||
| + | [[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology]][http://openwetware.org/images/4/48/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.pdf .]<br> | ||
| + | [[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology]] [http://openwetware.org/images/1/1c/Cell_Plating_for_Electrophysiology_SackLab.pdf .]<br> | ||
| + | [[Sack: Freezing CHO cells|Freezing CHO Cells]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf .]<BR> | ||
| + | [[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf .]<BR> | ||
| + | [[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Fluorescent Screening]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf .]<BR> | ||
| + | [[Sack: Subcloning Cells| Subcloning Cells]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf .]<BR> | ||
| + | [[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf .]<BR> | ||
| + | [[Sack: Thawing CHOcells| Thawing CHO cells]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf .]<BR> | ||
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| + | ==electrophys== | ||
| + | [[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf .]<br> | ||
| + | ==molecular biology== | ||
| + | [[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf .]<br> | ||
| + | Bacteria Transformation Invitrogen [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf .]<br> | ||
| + | Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf .]<br> | ||
| + | Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf .]<br> | ||
| + | ==solutions== | ||
| + | Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx .]<br> | ||
| + | Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx .]<br> | ||
| + | Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls .]<br> | ||
| + | Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls .]<br> | ||
| - | + | ==ELISAs== | |
| + | [[ELISA Protocol for a 96-well Plate]][http://openwetware.org/images/5/54/Elisa_protocol_for_online.pdf .]<br> | ||
| + | ELISA Spreadsheet Skeleton [http://openwetware.org/images/5/5b/Elisa_skeleton.xlsx .] | ||
Current revision
general labcell cultureCell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) . electrophysmolecular biology Bacteria Transformation (One Shot Top Ten Invitrogen). solutionsEphys Solutions 08/15/12. ELISAsELISA Protocol for a 96-well Plate. |
